背景:来自TCGA数据库的结果表明,与邻近组织相比,肺腺癌(LUAD)中磷脂酰肌醇特异性磷脂酶Cγ2(PLCG2)的表达水平显着降低,所以我们展示了LUAD的角色。
目的:本研究旨在探讨磷脂酰肌醇特异性磷脂酶Cγ2(PLCG2)在肺腺癌(LUAD)细胞中的表达及其在细胞增殖和转移中的作用。
方法:从TCGA数据库分析LUAD组织和邻近组织之间的差异PLCG2mRNA和蛋白水平,TIMER,和UALCAN数据库。通过R包以及GSEA对PLCG2mRNA高表达组和低表达组的患者进行差异表达基因的筛选。用qRT-PCR和CCK8检测PLCG2在LUAD细胞中的表达水平,Transwell,和蛋白质印迹分析。
结果:PLCG2在LUAD中低表达,与LUAD的预后无明显相关性。PLCG2表达水平在患者性别方面有显著差异,年龄,T,N,和病理阶段。GO/KEGG富集分析显示PLCG2的共表达主要与免疫反应调节细胞表面受体有关,等等。GSEA分析显示PLCG2相关差异基因的富集途径主要与嗅觉转导途径有关,核糖体,等。R软件分析显示PLCG2表达与6种类型的免疫浸润细胞之间存在显著的相关性,与免疫检查点相关基因呈正相关,受肿瘤突变负荷的负调控。过表达PLCG2显示LUAD细胞增殖减少,克隆形成,细胞迁移和侵袭,和上皮-间质转化相关蛋白,与对照组相比。
结论:PLCG2在LUAD组织中低表达,参与LUAD的免疫浸润,抑制LUAD细胞增殖和转移。
BACKGROUND: Results from the TCGA database showed that phosphatidylinositol-specific phospholipase Cγ2 (
PLCG2) expression level in Lung Adenocarcinoma (LUAD) was notably decreased compared to adjacent tissues, so we unveiled its role of LUAD.
OBJECTIVE: This study aims to explore the expression and clinical significance of Phosphatidyl-inositol-specific phospholipase Cγ2 (
PLCG2) in lung adenocarcinoma (LUAD) cells and its role in cell proliferation and metastasis.
METHODS: Differential
PLCG2 mRNA and protein levels between LUAD tissues and adjacent tissues were analyzed from the TCGA database, TIMER, and UALCAN database. Differentially expressed genes were screened for patients in the high and low PLCG2 mRNA expression groups by the R package as well as GSEA. The expression level of PLCG2 in LUAD cells was detected using qRT-PCR and CCK8, clone formation, Transwell, and Western blot assays.
RESULTS: PLCG2 was lowly expressed in LUAD and did not significantly correlate with the prognosis of LUAD. PLCG2 expression levels varied significantly in terms of patients\' gender, age, T, N, and pathological stage. GO/KEGG enrichment analysis showed that co-expression of
PLCG2 was mainly associated with the immune response- regulating cell-surface receptors, and so on. GSEA analysis showed enrichment pathways of
PLCG2-related differential gees were primarily associated with the olfactory transduction pathway, ribosome, etc. R software analysis revealed a significant correlation between PLCG2 expression and six types of immune-infiltrat-ing cells, positively correlated with immune checkpoint-related genes and negatively regulated by tumor mutational load. Overexpressing PLCG2 showed reduced LUAD cell proliferation, clone formation, cell migration and invasion, and epithelial-mesenchymal transition-associated proteins, compared with the control group.
CONCLUSIONS: PLCG2 is lowly expressed in LUAD tissues and is involved in immune infiltration of LUAD, inhibiting LUAD cell proliferation and metastasis.