未经批准:优化抗菌治疗以达到限制耐药性出现的药物暴露,有效治疗感染,降低副作用的风险在危重病人中尤为重要,其中正常功能增强或/和感染了对治疗不太敏感的病原体。这些目标的实现可以通过对许多抗生素的治疗药物监测(TDM)来增强。这里提出了一种液相色谱串联质谱(LC-MS/MS)方法,用于同时定量十种抗菌剂:头孢唑啉(CZO),头孢吡肟(CEP),头孢噻肟(CTA),头孢他啶(CTZ),环丙沙星(CIP),氟氯西林(FLU),利奈唑胺(LIN),美罗培南(MER),哌拉西林(PIP)和他唑巴坦(TAZ)在人血浆中。
未经证实:血浆样品用乙腈沉淀并注入LC-MS/MS。色谱分离在WatersAcquityBEHC18柱上进行。将化合物用水和含有0.1%甲酸的乙腈洗脱,使用梯度(0.5-65%B),在3.8分钟。流速为0.4毫升/分钟,运行时间为5.8min。
UNASSIGNED:校准曲线在测试浓度范围内呈线性(0.5-250,CZO,CEP,CTA,CTZ和FLU;0.2-100,MER和TAZ;0.1-50,CIP和LIN和1-500mg/L,PIP)。日内和日间不精确度<11%。准确度范围从95%到114%。CTZ和MER显示电离抑制,而CIP显示电离增强,使用内标进行标准化。
UNASSIGNED:开发了一种用于同时定量人血浆中十种抗微生物剂的LC-MS/MS方法,用于常规TDM。
UNASSIGNED: Optimizing antimicrobial therapy to attain drug exposure that limits the emergence of resistance, effectively treats the infection, and reduces the risk of side effects is of a particular importance in critically ill patients, in whom normal functions are augmented or/and are infected with pathogens less sensitive to treatment. Achievement of these goals can be enhanced by therapeutic drug monitoring (TDM) for many antibiotics. A liquid chromatography tandem mass spectrometry (LC-MS/MS) method is presented here for simultaneous quantification of ten antimicrobials: cefazolin (CZO), cefepime (CEP), cefotaxime (CTA), ceftazidime (CTZ), ciprofloxacin (CIP), flucloxacillin (FLU), linezolid (LIN), meropenem (MER), piperacillin (PIP) and tazobactam (TAZ) in human plasma.
UNASSIGNED: Plasma samples were precipitated with acetonitrile and injected into the LC-MS/MS. Chromatographic separation was on a Waters Acquity BEH C18 column. Compounds were eluted with water and acetonitrile containing 0.1 % formic acid, using a gradient (0.5-65 % B), in 3.8 min. The flow rate was 0.4 mL/min, and the run time was 5.8 min.
UNASSIGNED: The calibration curves were linear across the tested concentration ranges (0.5-250, CZO, CEP, CTA, CTZ and FLU; 0.2-100, MER and TAZ; 0.1-50, CIP and LIN and 1-500 mg/L, PIP). The intra and inter-day imprecision was < 11 %. Accuracy ranged from 95 to 114 %. CTZ and MER showed ionization suppression while CIP showed ionization enhancement, which was normalized with the use of the internal standard.
UNASSIGNED: An LC-MS/MS method for simultaneous quantification of ten antimicrobials in human plasma was developed for routine TDM.