迷走神经节,由迷走神经的上(颈)和下(颈)神经节组成,从头部和颈部接收体感信息,或者来自内脏器官的内脏感觉信息,分别。发展,颅神经c产生所有迷走神经胶质细胞和颈静脉神经节的神经元,而支气管上的胎盘产生了结节神经节的神经元。波峰衍生的结性神经胶质祖细胞还可以在周围神经系统中产生自主神经元,但是这些祖细胞如何产生神经元是未知的。这里,我们发现一些Sox10+神经c来源的细胞,和周围,结状神经节瞬时表达Phox2b,自主神经系统发育的主要调控者,在胚胎早期。我们在两种性别的小鼠中进行的遗传谱系追踪分析显示,尽管它们具有共同的发育起源和极端的空间接近性,但仍有相当大比例的神经胶质细胞。但不是在邻近的颈静脉神经节,有Phox2b表达的历史。我们使用单细胞RNA测序(scRNA-seq)来证明这些祖细胞在结节神经节中产生所有主要的神经胶质亚型,包括施万细胞,卫星神经胶质和神经胶质前体,并通过原位杂交绘制了它们的空间分布。最后,整合分析揭示了结节和背根神经节神经胶质亚型之间的转录组相似性,并显示出未成熟的结节胶质亚型。我们的工作表明,这些冠状神经胶质祖细胞瞬时表达Phox2b,产生整个互补的神经胶质细胞,并显示一个转录程序,可能是它们的双潜能性质的基础。意义陈述结节神经节包含神经支配内部器官的感觉神经元,并在消化等稳态行为中起关键作用,调节血压和心率,和呼吸。节点感觉神经元由节点神经胶质细胞支持,与它们的神经元邻居相比,它们的研究不足。具体来说,控制其发展的遗传程序尚未完全了解。这里,我们发现了一个独特的notose神经胶质细胞的转录程序(瞬时表达的Phox2b),解决了40岁的发现,即notose神经胶质祖细胞也可以产生自主神经神经元(其发育取决于Phox2b表达)。最后,我们利用单细胞RNA测序来鉴定结节性神经胶质细胞的四种主要亚型,并使用亚型特异性标记基因来绘制其空间分布图.
The vagal ganglia, comprised of the superior (jugular) and inferior (nodose) ganglia of the vagus nerve, receive somatosensory information from the head and neck or viscerosensory information from the inner organs, respectively. Developmentally, the cranial neural crest gives rise to all vagal glial cells and to neurons of the jugular ganglia, while the epibranchial placode gives rise to neurons of the nodose ganglia. Crest-derived nodose glial progenitors can additionally generate autonomic neurons in the peripheral nervous system, but how these progenitors generate neurons is unknown. Here, we found that some Sox10+ neural crest-derived cells in, and surrounding, the nodose ganglion transiently expressed
Phox2b, a master regulator of autonomic nervous system development, during early embryonic life. Our genetic lineage-tracing analysis in mice of either sex revealed that despite their common developmental origin and extreme spatial proximity, a substantial proportion of glial cells in the nodose, but not in the neighboring jugular ganglia, have a history of
Phox2b expression. We used single-cell RNA-sequencing to demonstrate that these progenitors give rise to all major glial subtypes in the nodose ganglia, including Schwann cells, satellite glia, and glial precursors, and mapped their spatial distribution by in situ hybridization. Lastly, integration analysis revealed transcriptomic similarities between nodose and dorsal root ganglia glial subtypes and revealed immature nodose glial subtypes. Our work demonstrates that these crest-derived nodose glial progenitors transiently express
Phox2b, give rise to the entire complement of nodose glial cells, and display a transcriptional program that may underlie their bipotent nature.