这项研究的重点是开发负载蜂毒(BV)并用PEG(BV-Lipo-PEG)包被的脂质体的最佳配方。使用动态光散射对脂质体进行表征,透射电子显微镜,和傅里叶变换红外光谱。在脂质体制剂中,F3表现出最窄的尺寸分布,具有193.72±7.35的低PDI值,表明最小的团聚相关问题和更均匀的尺寸分布。BV-Lipo-PEG在4°C下储存3个月后表现出显著的稳定性。此外,发现药物从脂质体制剂中的释放是pH依赖性的。此外,BV-Lipo-PEG表现出良好的包封效率,值达到96.74±1.49。脂质体纳米载体的抗癌潜力通过MTT试验进行评价,流式细胞术,细胞周期分析,和实时实验。脂质体系统的功能化增强了内吞作用。与游离药物和单独的BV-Lipo相比,BV-Lipo-PEG的IC50值显着降低,表明BV-Lipo-PEG在A549细胞系中更有效地诱导细胞死亡。与其他样品相比,BV-Lipo-PEG在A549细胞系中表现出更高的凋亡率。在用BV-Lipo-PEG处理的A549细胞系中,MMP-2、MMP-9和CyclinE基因表达水平降低,而Caspase3和Caspase9的表达水平增加。这些发现表明,通过聚乙二醇化脂质体递送BV对于肺癌的治疗具有重要的前景。
This study focused on developing an optimal formulation of liposomes loaded with bee venom (BV) and coated with
PEG (BV-Lipo-
PEG). The liposomes were characterized using dynamic light scattering, transmission electron microscopy, and Fourier transform infrared spectroscopy. Among the liposomal formulations, F3 exhibited the narrowest size distribution with a low PDI value of 193.72 ± 7.35, indicating minimal agglomeration-related issues and a more uniform size distribution. BV-Lipo-
PEG demonstrated remarkable stability over 3 months when stored at 4 °C. Furthermore, the release of the drug from the liposomal formulations was found to be pH-dependent. Moreover, BV-Lipo-PEG exhibited favorable entrapment efficiencies, with values reaching 96.74 ± 1.49. The anticancer potential of the liposomal nanocarriers was evaluated through MTT assay, flow cytometry, cell cycle analysis, and real-time experiments. The functionalization of the liposomal system enhanced endocytosis. The IC50 value of BV-Lipo-PEG showed a notable decrease compared to both the free drug and BV-Lipo alone, signifying that BV-Lipo-
PEG is more effective in inducing cell death in A549 cell lines. BV-Lipo-
PEG exhibited a higher apoptotic rate in A549 cell lines compared to other samples. In A549 cell lines treated with BV-Lipo-PEG, the expression levels of MMP-2, MMP-9, and Cyclin E genes decreased, whereas the expression levels of Caspase3 and Caspase9 increased. These findings suggest that delivering BV via PEGylated liposomes holds significant promise for the treatment of lung cancer.