先进的人工神经导管为神经损伤修复提供了一种有希望的替代方法。目前的研究集中在通过优化支架材料和功能部件来提高神经导管的治疗效果。在这项研究中,提出了一种新型的聚(3,4-亚乙基二氧噻吩)(PEDOT)整合的鱼泳囊(FSB)作为具有有序拓扑结构和电刺激的导电神经导管,以促进神经再生。PEDOT纳米材料和粘附肽(IKVAV)通过预涂覆聚多巴胺成功地掺入到脱细胞FSB底物上。获得的PEDOT/IKVAV集成FSB基材具有出色的机械性能,高导电性,稳定性,以及优异的生物相容性和生物粘附性能。体外研究证实,PEDOT/IKVAV整合的FSB可以有效促进嗜铬细胞瘤12细胞和背根神经节神经突的生长和定向延伸。此外,体内实验表明,所提出的PEDOT/IKVAV整合的FSB导管可以加速缺陷神经修复和功能恢复。研究结果表明,具有多个再生诱导信号整合的FSB衍生的导电神经导管为神经再生提供了有利的环境。显示出修复长段神经缺损的巨大潜力。
Advanced artificial nerve conduits offer a promising alternative for nerve injury repair. Current research focuses on improving the therapeutic effectiveness of nerve conduits by optimizing scaffold materials and functional components. In this study, a novel poly(3,4-ethylenedioxythiophene) (
PEDOT)-integrated fish swim bladder (FSB) is presented as a conductive nerve conduit with ordered topology and electrical stimulation to promote nerve regeneration.
PEDOT nanomaterials and adhesive peptides (IKVAV) are successfully incorporated onto the decellularized FSB substrate through pre-coating with polydopamine. The obtained
PEDOT/IKVAV-integrated FSB substrate exhibits outstanding mechanical properties, high electrical conductivity, stability, as well as excellent biocompatibility and bioadhesive properties. In vitro studies confirm that the
PEDOT/IKVAV-integrated FSB can effectively facilitate the growth and directional extension of pheochromocytoma 12 cells and dorsal root ganglion neurites. In addition, in vivo experiments demonstrate that the proposed
PEDOT/IKVAV-integrated FSB conduit can accelerate defective nerve repair and functional restoration. The findings indicate that the FSB-derived conductive nerve conduits with multiple regenerative inducing signals integration provide a conducive milieu for nerve regeneration, exhibiting great potential for repairing long-segment neural defects.