■观察不同BMI组AECOPD患者呼吸道菌群分布差异,探讨其对治疗的指导价值。
■收集38例AECOPD患者的痰标本。患者被分为低,正常和高BMI组。通过16SrRNA检测技术对痰菌群进行测序,并比较了痰菌群的分布。稀疏曲线,α-多样性,采用生物信息学方法对各组患者进行主坐标分析(PCoA)和痰菌群丰度测定分析.
■1.每个BMI组的稀疏曲线达到一个平台。各组微生物群的OTU总数或α多样性指数均无显著差异。PCoA显示三组间痰菌群的距离矩阵有显著差异,这是由二进制Jaccard和BrayCurtis算法计算的。2.在门一级,大多数微生物是变形杆菌,Firmicutes拟杆菌,放线菌,和镰状细菌。在属一级,大多数是链球菌,普雷沃氏菌,嗜血杆菌,奈瑟菌和拟杆菌。3.在门一级,低组的变形杆菌丰度明显高于正常组和高BMI组,低组和正常组的Firmicutes丰度明显低于高BMI组。在属一级,低组嗜血杆菌丰度显著高于高BMI组,低和正常BMI组的链球菌丰度明显低于高BMI组。
■1.不同BMI组AECOPD患者的痰菌群几乎覆盖了所有菌群,BMI与AECOPD患者呼吸道菌群总数和α-多样性无显著相关性。然而,不同BMI组间PCoA差异有统计学意义.2.不同BMI组AECOPD患者的菌群结构存在差异。低BMI组患者呼吸道中的革兰阴性菌(G-)占优势,而革兰氏阳性菌(G+)在高BMI组中占主导地位。
UNASSIGNED: To investigate the distribution differences in the respiratory tract microbiota of AECOPD patients in different BMI groups and explore its guiding value for treatment.
UNASSIGNED: Sputum samples of thirty-eight AECOPD patients were collected. The patients were divided into low, normal and high BMI group. The sputum microbiota was sequenced by 16S rRNA detection technology, and the distribution of sputum microbiota was compared. Rarefaction curve, α-diversity, principal coordinate analysis (
PCoA) and measurement of sputum microbiota abundance in each group were performed and analyzed by bioinformatics methods.
UNASSIGNED: 1. The rarefaction curve in each BMI group reached a plateau. No significant differences were observed in the OTU total number or α-diversity index of microbiota in each group.
PCoA showed significant differences in the distance matrix of sputum microbiota between the three groups, which was calculated by the Binary Jaccard and the Bray Curtis algorithm. 2. At the phylum level, most of the microbiota were Proteobacteria, Bacteroidetes Firmicutes, Actinobacteria, and Fusobacteria. At the genus level, most were Streptococcus, Prevotella, Haemophilus, Neisseria and Bacteroides. 3. At the phylum level, the abundance of Proteobacteria in the low group was significantly higher than that in normal and high BMI groups, the abundances of Firmicutes in the low and normal groups were significantly lower than that in high BMI groups. At the genus level, the abundance of Haemophilus in the low group was significantly higher than that in high BMI group, and the abundances of Streptococcus in the low and normal BMI groups were significantly lower than that in the high BMI group.
UNASSIGNED: 1. The sputum microbiota of AECOPD patients in different BMI groups covered almost all microbiota, and BMI had no significant association with total number of respiratory tract microbiota or α-diversity in AECOPD patients. However, there was a significant difference in the
PCoA between different BMI groups. 2. The microbiota structure of AECOPD patients differed in different BMI groups. Gram-negative bacteria (G-) in the respiratory tract of patients predominated in the low BMI group, while gram-positive bacteria (G+) predominated in the high BMI group.