背景:N-硝基吗啉(NMO)是最常见的N-亚硝基化合物之一。已在NMO治疗的大鼠的肾小管中证明了嗜酸细胞转化。在我们的研究中,我们旨在研究6个内分泌器官中嗜酸细胞的潜在转化,即,甲状腺,肾上腺和垂体,胰腺,睾丸,还有骨头,NMO暴露的老鼠。
方法:30只雄性大鼠出生饲养。其中15人给予每公斤体重320毫克NMO的单剂量,溶解在饮用水中,通过管胃管。在52周结束时,这两个系列的动物都被杀死了。就在杀戮之后,6种不同的内分泌器官(垂体,甲状腺,胰腺,肾上腺,骨[股骨],和睾丸)切除每只动物。
结果:对照组没有嗜酸性细胞发育的证据。相比之下,在13只NMO治疗的大鼠中,有8只观察到了肿瘤细胞:2只在肾上腺切片中,1在甲状腺切片中,3在垂体切片中,和2在胰腺部分。骨和骨切片完全正常。
结论:我们发现NMO诱导了胰腺的嗜酸细胞改变,甲状腺,垂体,和肾上腺.迄今为止,以前没有发现导致内分泌腺嗜酸细胞转化的特定环境风险因素的报道.鉴于环境中内分泌干扰化学物质的日益流行,个人护理产品,制成品,和食物来源,有必要提高我们对内分泌器官中嗜酸细胞病的病理机制的理解。
BACKGROUND: N-Nitrosomorpholine (NMO) is one of the most common N-nitroso compounds. An oncocytic transformation has been demonstrated in renal tubules of NMO-treated rats. In our study, we aimed to investigate the potential transformation of oncocytic cells in 6 endocrine organs, i.e., thyroid, adrenal and pituitary glands, pancreas, testis, and bone, of NMO-exposed rats.
METHODS: Thirty male rats were born and raised. Fifteen of them were given a single dose of 320 mg NMO per kg body weight, dissolved in drinking water, by a gavage tube. At the end of 52 weeks, the animals in both series were killed. Right after the killing, 6 different endocrine organs (hypophysis, thyroid, pancreas, adrenal gland, bone [femur], and testicles) of each animal were excised.
RESULTS: There was no evidence of oncocytic cell development in the control group. In contrast, oncocytes were observed in 8 out of 13 NMO-treated rats: 2 in the adrenal sections, 1 in the thyroid sections, 3 in the pituitary sections, and 2 in the pancreas sections. Thesticle and bone sections were completely normal.
CONCLUSIONS: We showed that NMO induced an oncocytic change in pancreas, thyroid, pituitary, and adrenal glands. To date, no identified specific environmental risk factors that lead to an oncocytic transformation in endocrine glands have been reported previously. Given the increasing prevalence of endocrine-disrupting chemicals in the environment, personal care products, manufactured goods, and food sources, there is a need to advance our understanding of the pathological mechanisms underlying oncocytosis in endocrine organs.