在这次调查中,我们使用正交离线二维RPLC×SFC方法成功地分离和纯化了天然二芳基庚类化合物，仅使用苯基/四唑固定相。首先,苯乙烯-二乙烯基苯基质介质预处理液相色谱系统有效处理了含叶绿素的植物提取液，回收率为33.8%，避免了浓缩步骤的需要。随后，仅使用苯基/四唑固定相的离线二维RPLC×SFC实现了显着的96.38％正交性，并被建立并用于天然产物的制备分离和纯化。最后,所构建的单固定相高度正交RPLC×SFC系统已成功应用于从虎耳草靶级分中分离和纯化天然二芳基庚类化合物，并获得了四种纯度超过95％的二芳基庚类化合物。
In this investigation, we successfully isolated and purified natural diarylheptanoids using an orthogonal offline two-dimensional RPLC × SFC approach, employing only the phenyl/tetrazole stationary phase. First, a styrene-divinylbenzene matrix medium pretreatment liquid chromatography system effectively processed chlorophyll-containing plant extract solution with a recovery rate of 33.8 %, obviating the need for concentration steps. Subsequently, an offline two-dimensional RPLC × SFC employing only the phenyl/tetrazole stationary phase achieved a remarkable 96.38 % orthogonality and was established and utilized in the preparative separation and purification of natural products. Finally, the constructed single stationary phase highly orthogonal RPLC × SFC system was successfully applied in the preparative separation and purification of natural diarylheptanoids from the Saxifraga tangutica target fraction and yielded four diarylheptanoids with purities exceeding 95 %.
