■立克次体和立克次体样疾病,由专性细胞内革兰氏阴性细菌产生,在中国构成了日益严重的公共卫生威胁。为了评估海南岛目前这些疾病的流行情况,使用TaqMan聚合酶链反应(TaqManPCR)阵列,对2018-2021年在海口接受治疗的未分化发热性疾病(UFI)患者中发现的9种细菌病原体进行了研究.
■从2018年至2021年的UFI患者收集血液样本(n=503)。筛选样品的立克次体属。,虫虫东方(O.tsum虫),解脲脲.吞噬细胞(A.吞噬细胞),查菲埃里希氏菌,伯内蒂柯西拉,披肩衣原体,布鲁氏菌属。,假伯克霍尔德菌,和伯氏疏螺旋体使用TaqManPCR阵列。阳性样本(Ct<35)通过巢式PCR进行确认,测序,测序和系统发育分析。
■O.在患者中检测到tsum虫和吞噬细胞A。阳性率分别为14.51%(73/503)和5.57%(28/503),分别。在来自海南的斑疹伤寒(ST)阳性人群中发现了O.tr虫和吞噬菌(10.96%,8/73),广西(61.54%,8/13),和云南(5.36%,3/56)中国省级行政区划(PLAD)。
■与十年前相比,海南UFI患者的ST患病率增加,立克次体病患病率降低。在中国,U.tsuppamushi和A.吞噬细胞的共同感染构成了当前的公共卫生威胁。
UNASSIGNED: Rickettsial and Rickettsial-like diseases, resulting from obligate intracellular Gram-negative bacteria, pose a growing public health threat in China. To assess the current prevalence of these diseases on Hainan Island, a study was conducted on 9 bacterial pathogens found in patients with undifferentiated febrile illness (UFI) treated in Haikou between 2018 and 2021 using a TaqMan Polymerase Chain Reaction (TaqMan PCR) array.
UNASSIGNED: Blood samples (n=503) were collected from patients with UFI between 2018 and 2021. The samples were screened for Rickettsia spp., Orientia tsutsugamushi (O. tsutsugamushi), Anaplasma. phagocytophilum (A. phagocytophilum), Ehrlichia chaffeensis, Coxiella burnetii, Chlamydia psittaci, Brucella spp., Burkholderia pseudomallei, and Borrelia burgdorferi using a TaqMan PCR array. Positive samples (Ct<35) underwent confirmation through nested PCR, sequencing, and phylogenetic analysis.
UNASSIGNED: O. tsutsugamushi and A. phagocytophilum were detected in the patients at positive rates of 14.51% (73/503) and 5.57% (28/503), respectively. Co-infection of O. tsutsugamushi and A. phagocytophilum was identified in scrub typhus (ST) positive populations from Hainan (10.96%, 8/73), Guangxi (61.54%, 8/13), and Yunnan (5.36%, 3/56) provincial-level administrative divisions (PLADs) of China.
UNASSIGNED: An increased prevalence rate of ST and a decreased prevalence of rickettsioses were observed in patients with UFI in Hainan compared to a decade ago. The co-infection of O. tsutsugamushi and A. phagocytophilum poses a current public health threat in China.