■红景天在亚洲和欧洲被用作促进健康的功能性食品。研究表明,红景天在动物模型中具有缓解炎症性肠病(IBD)的潜力。然而,改善肠道损伤的具体活性成分和潜在机制尚不清楚。本研究旨在探讨罗萨文(Rov)的缓解作用,一种已知的红景天活性成分,在IBD和监管机制方面。
■使用右旋糖酐硫酸钠盐(DSS)诱导的急性结肠炎的鼠模型评价Rov的治疗效果。通过相应的试剂盒测量炎性细胞因子和嗜中性粒细胞活化标志物。免疫组织化学,免疫荧光,TUNEL,和EdU分析用于研究紧密连接蛋白的表达,上皮标志物表达,凋亡细胞的数量,和上皮增殖,分别。使用TNF-α诱导的肠道类器官评估Rov对肠上皮损伤的保护作用。另外,应用RNA测序来观察这些肠类器官中的遗传改变谱。
■口服施用Rov显著减轻小鼠的体重减轻并恢复结肠长度。值得注意的是,Rov治疗导致促炎细胞因子和中性粒细胞活化标志物水平降低,同时增加抗炎因子。重要的是,Rov通过增加Lgr5+干细胞的数量恢复了肠道绝望,结肠炎小鼠肠道中的Lyz1+Paneth细胞和Muc2+杯状细胞,显示减少的上皮凋亡和恢复的屏障功能。在TNF-α诱导的肠道器官中,Rov促进上皮细胞分化并保护免受TNF-α诱导的损伤。RNA测序显示与上皮细胞相关的基因表达上调(包括Lgr5+,Lyz1+和Muc2+细胞)增殖和防御素分泌,揭示了Rov对肠上皮屏障的保护机制。
■Rov具有作为IBD天然预防剂的潜力,对肠上皮的保护作用对其治疗效果至关重要。
UNASSIGNED: Rhodiola species have been utilized as functional foods in Asia and Europe for promoting health. Research has demonstrated that Rhodiola has the potential to alleviate inflammatory bowel disease (IBD) in animal models. However, the specific active components and the underlying mechanism for ameliorating intestinal damage remain unclear. This study aims to explore the relieving effect of Rosavin (Rov), a known active constituent of Rhodiola, in IBD and the regulatory mechanisms.
UNASSIGNED: The therapeutic effect of Rov was evaluated using a murine model of acute colitis induced by dextran sulfate sodium salt (DSS). Inflammatory cytokines and neutrophil activation markers were measured by corresponding kits. Immunohistochemistry, immunofluorescence, TUNEL, and EdU assays were applied to investigate the tight conjunction proteins expression, epithelial marker expression, number of apoptotic cells, and epithelial proliferation, respectively. The protection effect of Rov on gut epithelial injury was assessed using TNF-α-induced intestinal organoids. Additinally, RNA sequencing was applied to observe the genetic alteration profile in these intestinal organoids.
UNASSIGNED: Oral administration of Rov significantly attenuated weight loss and restored colon length in mice. Notably, Rov treatment led to decreased levels of pro-inflammatory cytokines and neutrophil activation markers while increasing anti-inflammatory factors. Importantly, Rov restored intestinal despair by increasing the number of Lgr5+ stem cells, Lyz1+ Paneth cells and Muc2+ goblet cells in intestines of colitis mice, displaying reduced epithelial apoptosis and recovered barrier function. In TNF-α-induced intestinal organoids, Rov facilitated epithelial cell differentiation and protected against TNF-α-induced damage. RNA sequencing revealed upregulation in the gene expression associated with epithelial cells (including Lgr5+, Lyz1+ and Muc2+ cells) proliferation and defensin secretion, unveiling the protective mechanisms of Rov on the intestinal epithelial barrier.
UNASSIGNED: Rov holds potential as a natural prophylactic agent against IBD, with its protective action on the intestinal epithelium being crucial for its therapeutic efficacy.