近年来,基于有机荧光团的分子探针具有对体外/体内pH(DFR-MPs-pH)的双荧光比率响应,在基础应用研究领域引起了极大的兴趣。越来越多的科学出版物报道了各种DFR-MPs-pH系统的探索,这些系统具有独特的双荧光比率法作为信号输出,内置和信号自校准功能,提高目标的精确检测。DFR-MPs-pH系统在生物传感中具有高性能应用,生物成像和生物医学领域。这篇综述首次全面总结了DFR-MPs-pH的最新进展。首先,通过总结不同的基于有机荧光团的分子体系来介绍DFR-MPs-pH的组成和类型。然后,根据具体的组成部分分析施工策略,结构,DFR-MPs-pH的性质和功能。之后,详细讨论了生物传感和生物成像应用,主要是指活细胞和小动物水平的pH传感和成像检测。最后,全面总结生物医学应用,主要涉及生物毒性评估,生物分布,生物医学诊断和治疗。同时,当前状态,在对代表性和最新研究进行详细讨论后,对挑战和观点进行了理性评论。总的来说,这次审查是全面的,及时深入,并且可以促进以下对新的和通用的DFR-MPs-pH系统的进一步探索,朝着合理的设计,容易准备,优越的性能,可调的功能和高效的应用在有前途的领域。
In recent years, organic fluorophores-based molecular probes with dual-fluorescence ratiometric responses to in-vitro/in-vivo pH (DFR-MPs-pH) have been attracting much interest in fundamental application research fields. More and more scientific publications have reported the exploration of various DFR-MPs-pH systems that have unique dual-fluorescence ratiometry as the signal output, in-built and signal self-calibration functions to improve precise detection of targets. DFR-MPs-pH systems possess high-performance applications in biosensing, bioimaging and biomedicine fields. This review has comprehensively summarized recent advances of DFR-MPs-pH for the first time. First of all, the compositions and types of DFR-MPs-pH are introduced by summarizing different organic fluorophores-based molecule systems. Then, construction strategies are analyzed based on specific components, structures, properties and functions of DFR-MPs-pH. Afterward, biosensing and bioimaging applications are discussed in detail, primarily referring to pH sensing and imaging detection at the levels of living cells and small animals. Finally, biomedicine applications are fully summarized, majorly involving bio-toxicity evaluation, bio-distribution, biomedical diagnosis and therapeutics. Meanwhile, the current status, challenges and perspectives are rationally commented after detailed discussions of representative and state-of-the-art studies. Overall, this present review is comprehensive, in-time and in-depth, and can facilitate the following further exploration of new and versatile DFR-MPs-pH systems toward rational design, facile preparation, superior properties, adjustable functions and highly efficient applications in promising fields.