背景:口腔真菌群的菌群失调与一些疾病有关,包括癌症。然而,口腔真菌群落在鼻咽癌(NPC)癌变中的作用以前尚未得到研究.
方法:我们使用真菌内部转录间隔区(ITS)-2测序,在476名未经治疗的NPC患者和537名基于人群的对照中,对口腔唾液真菌真菌基因组进行了表征。通过生物信息学和生物统计学分析评估口腔真菌基因组与NPC风险之间的关系。
结果:我们发现较低的真菌α多样性与NPC的几率增加有关[较低与较高:观察到的特征(调整后的比值比[OR]=5.81,95%置信区间[CI]=3.60-9.38);辛普森多样性(1.53,1.03-2.29);香农多样性(2.03,1.35-3.04)]。我们还观察到,基于Bray-Curtis差异,病例和对照组之间的全球真菌群落模式存在显着差异(P<0.001)。口腔真菌物种的运输,具体来说,酿酒酵母,热带念珠菌,长孢子菌,白色念珠菌,和镰刀菌,与显著较高的NPC几率相关,OR范围从1.56到4.66。真菌和细菌α多样性低的个体患NPC的风险显著升高。
结论:我们的结果表明口腔真菌群的菌群失调,以真菌群落多样性的丧失和几种真菌有机体的过度生长为特征,与NPC的风险大幅增加有关。
背景:这项工作由美国国立卫生研究院资助,瑞典研究委员会,福建医科大学高层次人才研究启动项目,和中国奖学金委员会。
BACKGROUND: Dysbiosis of the oral mycobiome has been linked to some diseases, including cancers. However, the role of oral fungal communities in nasopharyngeal carcinoma (NPC) carcinogenesis has not previously been investigated.
METHODS: We characterized the oral salivary fungal mycobiome in 476 untreated incident NPC patients and 537 population-based controls using fungal internal transcribed spacer (ITS)-2 sequencing. The relationship between oral fungal mycobiome and the risk of NPC was assessed through bioinformatic and biostatistical analyses.
RESULTS: We found that lower fungal alpha diversity was associated with an increased odds of NPC [lower vs. higher: observed features (adjusted odds ratio [OR] = 5.81, 95% confidence interval [CI] = 3.60-9.38); Simpson diversity (1.53, 1.03-2.29); Shannon diversity (2.03, 1.35-3.04)]. We also observed a significant difference in global fungal community patterns between cases and controls based on Bray-Curtis dissimilarity (P < 0.001). Carriage of oral fungal species, specifically, Saccharomyces cerevisiae, Candida tropicalis, Lodderomyces elongisporus, Candida albicans, and Fusarium poae, was associated with significantly higher odds of NPC, with ORs ranging from 1.56 to 4.66. Individuals with both low fungal and low bacterial alpha diversity had a profoundly elevated risk of NPC.
CONCLUSIONS: Our results suggest that dysbiosis in the oral mycobiome, characterized by a loss of fungal community diversity and overgrowth of several fungal organisms, is associated with a substantially increased risk of NPC.
BACKGROUND: This work was funded by the US National Institutes of Health, the Swedish Research Council, the High-level Talents Research Start-up Project of Fujian Medical University, and the China Scholarship Council.