■特应性皮炎(AD)是皮肤科临床常见的复发性特应性疾病,多见于儿童和青少年。近年来,已发现AD与微生物群落密切相关。
■为了探索来自不同部位的定植细菌与AD之间的协同作用,我们比较分析了皮肤,口服,通过16SrRNA基因测序,对患有AD的儿童(50例)和健康儿童(50例)的肠道微生物群进行分析。还从两组中随机选择20个样品进行代谢和大基因组测序。
■我们的测序研究结果表明口腔中的微生物群多样性减少,皮肤,与AD患儿肠道有关(P<0.05)。代谢组学分析表明,5-羟色胺能突触,花生四烯酸代谢,类固醇生物合成在口服的所有三个位点都下调,皮肤,与AD患儿肠道有关(P<0.05)。宏基因组测序分析表明,三个位点菌群的KEGG功能通路参与了氧化磷酸化,泛素介导的蛋白水解,mRNA监测途径,真核生物的核糖体生物发生,蛋白酶体,基础转录因子,过氧化物酶体,MAPK信号通路,线粒体自噬,脂肪酸伸长,依此类推(P<0.05)。
■组合的微生物,新陈代谢,宏观遗传学分析确定了关键细菌,代谢物,和可能与AD发展相关的致病途径。我们对AD患者不同部位的微生物群的作用提供了更全面和深入的了解,指出了未来诊断的新方向,治疗和预后。
UNASSIGNED: Atopic dermatitis (AD) is a common clinical recurrent atopic disease in dermatology, most seen in children and adolescents. In recent years, AD has been found to be closely associated with microbial communities.
UNASSIGNED: To explore the synergistic effects between colonizing bacteria from different sites and AD, we comparatively analyzed the skin, oral, and gut microbiota of children with AD (50 individuals) and healthy children (50 individuals) by 16S rRNA gene sequencing. Twenty samples were also randomly selected from both groups for metabolic and macrogenomic sequencing.
UNASSIGNED: The results of our sequencing study showed reduced microbiota diversity in the oral, skin, and gut of children with AD (P < 0.05). Metabolomics analysis showed that serotonergic synapse, arachidonic acid metabolism, and steroid biosynthesis were downregulated at all three loci in the oral, skin, and gut of children with AD (P < 0.05). Macrogenomic sequencing analysis showed that KEGG functional pathways of the three site flora were involved in oxidative phosphorylation, ubiquitin-mediated proteolysis, mRNA surveillance pathway, ribosome biogenesis in eukaryotes, proteasome, basal transcription factors, peroxisome, MAPK signaling pathway, mitophagy, fatty acid elongation, and so on (P < 0.05).
UNASSIGNED: The combined microbial, metabolic, and macrogenetic analyses identified key bacteria, metabolites, and pathogenic pathways that may be associated with AD development. We provides a more comprehensive and in-depth understanding of the role of the microbiota at different sites in AD patients, pointing to new directions for future diagnosis, treatment and prognosis.