目的:口腔癌的发病机制复杂。我们检验了口腔微生物群失调与口腔癌相关的假设。
方法:符合纳入和排除标准的原发性口腔癌患者被纳入研究。招募匹配的健康个体作为对照。关于社会人口和行为因素的数据,自我报告的牙周措施和习惯,使用结构化问卷和牙周图表收集当前的牙齿状况。除了自我报告的口腔健康措施,每位参与者都接受了标准和详细的临床检查.从患者和健康对照的唾液样品中提取DNA。通过靶向16SrRNA的V3-V4基因区域进行下一代测序,随后进行生物信息学分析。
结果:口腔癌患者的口腔健康质量低于健康对照组。变形杆菌,Aggregatibacter,嗜血杆菌,奈瑟菌减少了,而Firmicutes,拟杆菌,放线菌,乳酸菌,Gemella,口腔癌患者的梭菌增多。在物种层面,C.硬粒,L.umeaens,N.subflava,A.massiliensis,V.dispar明显较低,而G.huolysans显著增加(p<0.05)。与牙周病相关的主要牙周病原体(P。牙龈和核仁F.)增加6.5和2.8倍,分别。
结论:这些数据表明,口腔癌患者的口腔健康状况较差,口腔微生物组组成明显,受个人日常习惯的影响,可能与疾病的致病性和种间相互作用有关。
结论:本文证明了口腔细菌与口腔癌之间的联系,确定口腔微生物组物种之间的机械相互作用。
OBJECTIVE: The pathogenesis of oral cavity cancers is complex. We tested the hypothesis that oral microbiota dysbiosis is associated with oral cavity cancer.
METHODS: Patients with primary oral cavity cancer who met the inclusion and exclusion criteria were included in the study. Matching healthy individuals were recruited as controls. Data on socio-demographic and behavioral factors, self-reported periodontal measures and habits, and current dental status were collected using a structured questionnaire and periodontal chartings. In addition to self-reported oral health measures, each participant received a standard and detailed clinical examination. DNA was extracted from saliva samples from patients and healthy controls. Next-generation sequencing was performed by targeting V3-V4 gene regions of the 16 S rRNA with subsequent bioinformatic analyses.
RESULTS: Patients with oral cavity cancers had a lower quality of oral health than healthy controls. Proteobacteria, Aggregatibacter, Haemophilus, and Neisseria decreased, while Firmicutes, Bacteroidetes, Actinobacteria, Lactobacillus, Gemella, and Fusobacteria increased in oral cancer patients. At the species level, C. durum, L. umeaens, N. subflava, A. massiliensis, and V. dispar were significantly lower, while G. haemolysans was significantly increased (p < 0.05). Major periodontopathogens associated with periodontal disease (P. gingivalis and F.nucleatum) increased 6.5- and 2.8-fold, respectively.
CONCLUSIONS: These data suggested that patients with oral cancer had worse oral health conditions and a distinct oral microbiome composition that is affected by personal daily habits and may be associated with the pathogenicity of the disease and interspecies interactions.
CONCLUSIONS: This paper demonstrates the link between oral bacteria and oral cancers, identifying mechanistic interactions between species of oral microbiome.