重氮营养结核分离株是公认的植物生长和根际群落的启动子。因此,在小扁豆农业生态系统中,接种非典型根瘤菌菌株可能是优化休耕土地利用的化学肥料的可行替代方法。这项研究的目的是评估和选择具有植物生长促进(PGP)属性的扁豆根瘤的根瘤菌分离株,并阐明其在水稻休耕土壤中的应用,以确定扁豆的生长及其对根际细菌的影响。群落。对小扁豆的结节分离株进行鉴定,并筛选其PGP属性,生物膜,胞外多糖(EPS)的形成,和早期植物生长促进。使用选定的非典型根瘤菌分离株副根瘤菌(P1)和苍白杆菌属进行盆栽实验。(42S)显著增强发芽,活力指数,结节形成(P160%,42S增加42%),结节鲜重,拍摄长度(65%P1和35%42S),和叶绿素含量与未接种对照处理相比。在两个分离株中均检测到固氮基因nifH和nifK。扫描电子显微镜(SEM)显示两个分离株成功的根和结节定植,而透射电子显微镜(TEM)显示根瘤内的固氮区。在所有治疗方法中,小扁豆根际中的变形杆菌占主导地位。然而,施用P1或42S增加根瘤菌,中根瘤菌,和根草根瘤菌,从而积极调节根际群落结构。相关网络分析显示,一些相互依存的细菌属丰富,可能在整体植物生长中起作用。通过应用P1或42S,可以积极调节铁载体生物合成和ABC转运蛋白的功能基因。这项研究表明,加那霉素P1和苍白杆菌属具有显着的作用。42S对小扁豆生长的影响,改善退化土壤健康,以实现最佳使用,和调制的根际群落结构,强烈显示出低成本的前景,环保和可持续的生物肥料。
Diazotrophic nodule isolates are acknowledged promoters of plant growth and rhizospheric community. Consequently, in the lentil agroecosystem, inoculation of atypical rhizobial isolates could be a viable alternative to chemical fertilizers for fallow land usage optimization. The aim of this study is to evaluate and select the rhizobial isolates of lentil nodules with plant-growth-promoting (PGP) attributes and to elucidate their application in rice-fallow soil for determining the growth of lentils and its impact on the rhizospheric bacterial community. Lentil\'s nodule isolates were identified and screened for their PGP attributes, biofilm, exopolysaccharide (EPS) formation, and early plant growth promotion. The pot experiment with the selected atypical rhizobial isolates Pararhizobium giardinii (P1) and Ochrobactrum sp. (42S) significantly enhanced germination, vigour index, nodule formation (P1 60%, 42S 42% increase), nodule fresh weight, shoot length (65% P1 & 35% 42S), and chlorophyll content as compared to the uninoculated control treatment. The genes for nitrogen fixation nifH and nifK were detected in both isolates. Scanning Electron Microscopy (SEM) revealed successful root and nodule colonization by both isolates, while Transmission Electron Microscopy (TEM) displayed nitrogen-fixing zones within root nodules. Proteobacteria predominated in the lentil rhizosphere of all the treatments. Whereas, application of either P1 or 42S increased Rhizobium, Mesorhizobium, and Bradyrhizobium genra, thus positively modulating rhizospheric community structure. The correlation network analysis revealed an abundance of some interdependent bacterial genera with a possible role in overall plant growth. Functional genes for siderophore biosynthesis and ABC transporter were positively modulated by application of either P1 or 42S. This study showed the significant effect of P. giardinii P1 and Ochrobactrum sp. 42S of L. culinaris on lentil growth, improving fallowsoil health for optimum usage, and modulated rhizospheric community structure which strongly manifest prospects of low-cost, eco-friendly and sustainable biofertilizers.