隐匿性乙型肝炎病毒(HBV)感染(OBI)是HBV预防和控制的重大挑战。我们调查了湖州市献血者中OBI的患病率和表面(S)基因突变,中国东部。在44,256名献血者中常规筛查乙型肝炎表面抗原(HBsAg)。使用Rochecobas®系统检测HBV-DNA。选择HBsAg阴性和HBV-DNA阳性的血清样本,并对HBVS基因进行扩增和测序。分析HBV基因型和S基因突变。这些献血者的OBI率为0.070%(31/44,256)。在OBI的献血者中,只有2例(2/31,6.5%)抗-HBc阴性。成功获得28个样本的S基因序列,我们发现HBV基因型C(21/28,70%)在OBI献血者中占主导地位。大多数S基因突变与OBI相关,高频突变包括N40S,G44E,Q51R/P,T113A/S,T118K/M,P120Q/S/T,Y161F/S值得注意的是,某些位点的氨基酸取代与以前报道的不同,例如Y72F,G102V,P127L,Q129P,和S143T。此外,六个新突变(S31I/N/R,P46L,S58C,C76Y,Y200F/C,发现可能与OBI相关的I208T)。湖州市有一定比例的献血者检出OBI。S基因突变在OBI发生发展中起重要作用。新S基因突变体在OBI发病机制中的功能有待进一步研究。这项研究的结果可能提供重要的见解,以防止HBV通过输血传播。
Occult hepatitis B virus (HBV) infection (OBI) is a significant challenge for HBV prevention and control. We investigated the prevalence and surface (S) gene mutations of OBI among blood donors in Huzhou City, eastern China. The hepatitis B surface antigen (HBsAg) was routinely screened among 44,256 blood donors. HBV-DNA was detected using the Roche cobas®system. Serum samples that were HBsAg negative and HBV-DNA positive were selected, and the HBV S gene was amplified and sequenced. HBV genotype and S gene mutations were analyzed. The OBI rate in these blood donors was 0.070 % (31/44,256). Among the blood donors with OBI, only two cases (2/31, 6.5 %) were anti-HBc negative. The S gene sequences of 28 samples were successfully obtained, and we found that HBV genotype C (21/28, 70 %) was predominant among blood donors with OBI. Most S gene mutations were associated with OBI, and the high frequency mutations included N40S, G44E, Q51R/P, T113A/S,T118K/M, P120Q/S/T, and Y161F/S. Notably, amino acid substitutions at some sites differed from those reported previously, such as Y72F, G102V, P127L, Q129P, and S143T. Additionally, six novel mutations (S31I/N/R, P46L, S58C, C76Y, Y200F/C, and I208T) that may be associated with OBI were found. OBI was detected in a certain proportion of blood donors in Huzhou City. S gene mutations play an important role in OBI development. Further research is required to explore the functions of novel S gene mutants in OBI pathogenesis. The findings of this study may provide important insights to prevent HBV transmission through blood transfusions.