背景:目前尚不清楚长链非编码RNA(lncRNA)OTX2反义RNA1(OTX2-AS1)是否在胃癌(GC)中起关键作用。通过对癌症基因组图谱(TCGA)数据库数据和生物信息学的分析,探讨了本研究中OTX2-AS1与GC之间的关系。
方法:我们评估了临床特征与OTX2-AS1表达之间的关系,预后因素,使用各种统计方法,OTX2-AS1在功能上的显著参与,例如Kaplan-Meier方法,Cox回归分析,基因集富集分析(GSEA),和免疫浸润分析。使用qRT-PCR测试GC细胞系的OTX2-AS1表达。
结果:在GC患者中,高水平的OTX2-AS1表达与幽门螺杆菌(Hpylori)感染显着负相关(P=.006),并预测总体生存期(OS)较差(HR:1.54;95%CI:1.10-2.14;P=.011),GC患者的无进展间期(PFI)(HR:1.75;95%CI:1.22-2.51;P=.002)和疾病特异性生存期(DSS)(HR:1.85;95%CI:1.21-2.85;P=.005).GC患者OTX2-AS1表达(HR:1.771;95%CI:1.164-2.696;P=.008)与OS之间存在独立相关性。OTX2-AS1高表达表型在嗅觉转导中存在差异富集,Gα信号事件,角质化,嗅觉信号通路,和植入前胚胎。OTX2-AS1的表达可能与某些免疫浸润细胞有关。与胃上皮细胞(GES-1)相比,GC细胞系显示OTX2-AS1表达显著增加。
结论:GC患者的OTX2-AS1表达与低生存率之间存在显著关联,提示它可能是胃腺癌(STAD)GC预后和免疫治疗结果的有用生物标志物。
It is unclear whether the long non-coding RNA (lncRNA) OTX2 antisense RNA 1 (OTX2-AS1) plays a pivotal role in gastric cancer (GC). An analysis of The Cancer Genome Atlas (TCGA) database data and bioinformatics was used to explore the relationship between OTX2-AS1 and GC in the current study.
We evaluated the relationship between clinical features and OTX2-AS1 expression, prognostic factors, and the significant involvement of OTX2-AS1 in function using various statistical methods, such as Kaplan-Meier method, Cox regression analysis, Gene Set Enrichment Analysis (GSEA), and immune infiltration analysis. GC cell lines were tested for OTX2-AS1 expression using qRT-PCR.
A high level of OTX2-AS1 expression was significantly and negatively associated with Helicobacter pylori (H pylori) infection in GC patients (P = .006) and predicted a poorer overall survival (OS) (HR: 1.54; 95% CI: 1.10-2.14; P = .011), progression-free interval (PFI) (HR: 1.75; 95% CI: 1.22-2.51; P = .002) and disease-specific survival (DSS) (HR: 1.85; 95% CI: 1.21-2.85; P = .005) in GC patients. There was an independent correlation between OTX2-AS1 expression (HR: 1.771; 95% CI: 1.164-2.696; P = .008) and OS in patients with GC. There were differential enrichments for the OTX2-AS1 high expression phenotype in the olfactory transduction, G alpha (s) signaling events, keratinization, olfactory signaling pathway, and preimplantation embryo. OTX2-AS1 expression may be related to certain immune-infiltrating cells. Compared to gastric epithelial cells (GES-1), GC cell lines showed a significant increase in OTX2-AS1 expression.
There was a significant association between OTX2-AS1 expression in GC patients and poor survival, suggesting that it may be a useful biomarker for prognosis and immunotherapy outcome of stomach adenocarcinoma (STAD) in GC.