■OAS1(2'-5'-寡腺苷酸合成酶1)是干扰素刺激基因的成员,在抗病毒过程中起重要作用。近年来,OAS1在肿瘤中的作用引起了人们的关注,发现它与几种肿瘤的预后有关。然而,OAS1影响肿瘤的机制尚不清楚,因此有必要对OAS1进行泛癌症研究,以更好地了解其在癌症中的意义.
■表达式,预后价值,遗传改变,使用TCGA分析泛癌中OAS1的选择性剪接事件,GTEx,HPA,GEPIA和OncoSplicing数据库。使用ESTIMATE评估OAS1相关的免疫细胞浸润,xCell,CIBERSORT和QUANTISEQ算法。使用TISH数据库下载单细胞转录组数据。最后,OAS1对细胞凋亡的作用,研究了两种胰腺癌细胞的迁移和侵袭。
■我们的结果表明,OAS1在各种肿瘤中的表达存在显着差异,具有预后意义。此外,我们研究了OAS1对基因组稳定性的影响,甲基化状态,以及不同类型癌症的其他因素,以及这些因素对预后的影响。值得注意的是,我们的研究还表明,OAS1过表达可能导致CTL功能障碍和巨噬细胞M2极化。此外,细胞实验表明,敲低OAS1可以降低PAAD细胞的侵袭能力,增加细胞凋亡率。
■这些结果证实了OAS1在CTL功能障碍和巨噬细胞M2极化中的潜在作用可能是预后生物标志物和治疗靶标。
OAS1(2\'-5\'-oligoadenylate synthetase 1) is a member of the Interferon-Stimulated Genes which plays an important role in the antiviral process. In recent years, the role of
OAS1 in tumors has attracted attention, and it was found to be associated with prognosis in several tumors. However, the mechanism by which
OAS1 affects tumors is unclear and pan-cancer study of
OAS1 is necessary to better understand its implication in cancers.
The expression, prognostic value, genetic alteration, alternative splicing events of OAS1 in pan-cancers were analyzed using TCGA, GTEx, HPA, GEPIA and OncoSplicing databases. OAS1 associated immune cell infiltration was evaluated using the ESTIMATE, xCell, CIBERSORT and QUANTISEQ algorithm. Single cell transcriptome data download using TISH database. Finally, the roles of the
OAS1 on apoptosis, migration and invasion were investigated in two pancreatic cancer cells.
Our results revealed significant differences in OAS1 expression among various tumors, which had prognostic implications. In addition, we investigated the impact of OAS1 on genomic stability, methylation status, and other factors across different types of cancer, and the effects of these factors on prognosis. Notably, our study also demonstrated that OAS1 overexpression can contribute to CTL dysfunction and macrophage M2 polarization. In addition, cell experiments showed that the knockdown of
OAS1 could reduce the invasive ability and increased the apoptosis rate of PAAD cells.
These results confirmed that OAS1 could be a prognostic biomarker and therapeutic target for its potential role in CTL dysfunction and macrophage M2 polarization.