肺炎克雷伯菌由于其多药耐药性和不同血清型景观而提出了重大的医疗保健挑战。本研究旨在探讨1072例肺炎克雷伯菌的血清型多样性及其与地理分布的关系。印度的疾病严重程度和抗菌/毒力模式。在Illumina平台上进行全基因组测序,使用Kleborate工具进行基因组分析。分析揭示了总共78种不同的KL类型,其中KL64(n=274/1072,26%),KL51(n=249/1072,24%),KL2(n=88/1072,8%)最为普遍。相比之下,仅鉴定出13种不同的O型,O1/O2v1(n=471/1072,44%),O1/O2v2(n=353/1072,33%),OL101(n=66/1072,6%)是主要的血清型。该研究鉴定了114种具有不同血清型的不同序列类型(STs),ST231是最主要的。O血清型与STs密切相关,O1/O2v1主要与ST231相关。辛普森的多样性指数和费舍尔的精确检验显示,北部和东部地区的血清型多样性较高,以及特定血清型和抗性谱之间的有趣关联。未观察到KL或O型与疾病严重程度之间的显著关联。此外,我们发现毒力因子耶尔森纳汀和aerobactin与KL类型有特异性关联(P<0.05),而与O抗原类型无关(P>0.05)。印度常规描述的高毒力克隆(即KL1和KL2)缺乏典型的毒力标记(即aerobactin),与其他区域血清型(KL51)形成对比。KL和O血清型的累积分布表明,未来的疫苗可能必须包括〜20KL或四种O类型,以覆盖>85%的产碳青霉烯酶的印度肺炎克雷伯菌群体。结果强调了采取综合策略来管理印度不同地区肺炎克雷伯菌菌株的多样化景观的必要性。了解区域血清型动态对于有针对性的监测至关重要,干预措施,和量身定制的疫苗策略,以应对印度肺炎克雷伯菌感染的多样化局面。本文包含Microreact托管的数据。
Klebsiella pneumoniae poses a significant healthcare challenge due to its multidrug resistance and diverse serotype landscape. This study aimed to explore the serotype diversity of 1072 K. pneumoniae and its association with geographical distribution, disease severity and antimicrobial/virulence patterns in India. Whole-genome sequencing was performed on the Illumina platform, and genomic analysis was carried out using the Kleborate tool. The analysis revealed a total of 78 different KL types, among which KL64 (n=274/1072, 26 %), KL51 (n=249/1072, 24 %), and KL2 (n=88/1072, 8 %) were the most prevalent. In contrast, only 13 distinct O types were identified, with O1/O2v1 (n=471/1072, 44 %), O1/O2v2 (n=353/1072, 33 %), and OL101 (n=66/1072, 6 %) being the predominant serotypes. The study identified 114 different sequence types (STs) with varying serotypes, with ST231 being the most predominant. O serotypes were strongly linked with STs, with O1/O2v1 predominantly associated with ST231. Simpson\'s diversity index and Fisher\'s exact test revealed higher serotype diversity in the north and east regions, along with intriguing associations between specific serotypes and resistance profiles. No significant association between KL or O types and disease severity was observed. Furthermore, we found the specific association of virulence factors yersiniabactin and aerobactin (P<0.05) with KL types but no association with O antigen types (P>0.05). Conventionally described hypervirulent clones (i.e. KL1 and KL2) in India lacked typical virulent markers (i.e. aerobactin), contrasting with other regional serotypes (KL51). The cumulative distribution of KL and O serotypes suggests that future vaccines may have to include either ~20 KL or four O types to cover >85 % of the carbapenemase-producing Indian K. pneumoniae population. The results highlight the necessity for comprehensive strategies to manage the diverse landscape of K. pneumoniae strains across different regions in India. Understanding regional serotype dynamics is pivotal for targeted surveillance, interventions, and tailored vaccine strategies to tackle the diverse landscape of K. pneumoniae infections across India. This article contains data hosted by Microreact.