目的:本研究的目的是研究白细胞介素(IL)-1β,IL-18,节点样受体pyrin结构域含蛋白3(NLRP3),含有caspase激活和募集结构域(ASC)的凋亡相关斑点样蛋白,不同牙周疾病患者唾液和血清中caspase-1水平的变化,并评估非手术牙周治疗(NSPT)后的变化。
方法:共有45名参与者,15健康,15牙龈炎,和15个III期C级(SIIIGC)牙周炎患者,包括在研究中。评估牙周参数,在所有组的基线以及牙龈炎和牙周炎组的NSPT后1个月和3个月收集唾液和血清样本。酶联免疫吸附试验用于分析IL-1β,IL-18,NLRP3,ASC,和caspase-1水平。
结果:在NSPT之后,在所有临床参数中观察到改善,牙龈炎和牙周炎组的牙周发炎表面积(PISA)。PISA评分与IL-1β呈正相关,NLRP3和caspase-1在基线(p<0.05)。唾液和血清IL-1β,与健康对照相比,基线时牙周炎中的NLRP3水平较高,治疗后降低(p<0.05)。接收器工作特征分析显示,唾液IL-1β,NLRP3和caspase-1具有区分SIIIGC牙周炎患者与健康受试者的能力(p<0.05)。
结论:结论:唾液IL-1β,NLRP3和caspase-1在SIIIGC牙周炎中处于异常高水平,并且在NSPT后显着降低;这些炎症小体生物标志物可能在诊断和监测牙周炎中显示出潜在的效用。
OBJECTIVE: The purpose of this study was to investigate interleukin (IL)-1β, IL-18, nod-like receptor pyrin domain-containing protein 3 (NLRP3), apoptosis-related speck-like protein containing a caspase activation and recruitment domain (ASC), and caspase-1 levels in saliva and serum in different periodontal diseases and to evaluate the changes after non-surgical periodontal treatment (NSPT).
METHODS: A total of 45 participants, 15 healthy, 15 gingivitis, and 15 stage III grade C (SIIIGC) periodontitis patients, were included in the study. Periodontal parameters were assessed, and salivary and serum samples were collected at baseline in all groups and one and three months after NSPT in gingivitis and periodontitis groups. An enzyme-linked immunosorbent assay was used to analyse IL-1β, IL-18, NLRP3, ASC, and caspase-1 levels.
RESULTS: After NSPT, improvement was observed in all clinical parameters, along with periodontal inflamed surface area (PISA) in gingivitis and periodontitis groups. PISA scores were positively correlated with IL-1β, NLRP3, and caspase-1 at baseline (p < 0.05). Salivary and serum IL-1β, NLRP3 levels were higher in periodontitis compared to healthy controls at baseline and reduced after treatment (p < 0.05). Receiver operating characteristic analysis revealed that salivary IL-1β, NLRP3, and caspase-1 had the ability to discriminate SIIIGC periodontitis patients from healthy subjects (p < 0.05).
CONCLUSIONS: In conclusion, salivary IL-1β, NLRP3, and caspase-1 are at aberrantly high levels in SIIIGC periodontitis and are remarkably decreased following NSPT; these inflammasome biomarkers may show potential utility in diagnosing and monitoring periodontitis.