■无标签非线性光学显微镜已成为生物医学研究的有力工具。然而,可能的光损伤风险阻碍了进一步的临床应用。
■为了减少这些不利影响,我们构建了一个新的同步无标记自发荧光多谐波(SLAM)显微镜平台,具有四通道多模态成像,内联光损伤监测,和脉冲重复率调谐。
■使用大芯双折射光子晶体光纤进行光谱展宽,并使用棱镜压缩器进行脉冲预chi,该系统允许用户独立调整脉冲宽度,重复率,和能量,这对于优化成像条件朝向无/最小光损伤是有用的。
■它演示了在每个图像像素一个激发脉冲下的无标签多通道成像,从而为通过更快的光学扫描仪提高成像速度铺平了道路,该扫描仪具有较低的非线性光损伤风险。此外,该系统赋予用户自主微调重复率的灵活性,脉冲宽度,平均功率,不受干扰,确保在各种应用中发现具有高SNR和最小光毒性的最佳成像条件。
■稳定激光源的组合,独立可调超短脉冲,光损伤监测功能,紧凑的设计使这个新系统变得强大,强大,和用户友好的成像平台。
UNASSIGNED: Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinders further clinical applications.
UNASSIGNED: To reduce these adverse effects, we constructed a new platform of simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy, featuring four-channel multimodal imaging, inline photodamage monitoring, and pulse repetition-rate tuning.
UNASSIGNED: Using a large-core birefringent photonic crystal fiber for spectral broadening and a prism compressor for pulse pre-chirping, this system allows users to independently adjust pulse width, repetition rate, and energy, which is useful for optimizing imaging conditions towards no/minimal photodamage.
UNASSIGNED: It demonstrates label-free multichannel imaging at one excitation pulse per image pixel and thus paves the way for improving the imaging speed by a faster optical scanner with a low risk of nonlinear photodamage. Moreover, the system grants users the flexibility to autonomously fine-tune repetition rate, pulse width, and average power, free from interference, ensuring the discovery of optimal imaging conditions with high SNR and minimal phototoxicity across various applications.
UNASSIGNED: The combination of a stable laser source, independently tunable ultrashort pulse, photodamage monitoring features, and a compact design makes this new system a robust, powerful, and user-friendly imaging platform.