背景:由于缺乏准确表明老年人每天应该采取多少步骤进行抗氧化防御的研究,骨代谢,和认知能力的提高,我们的研究开始比较选定的抗氧化剂,促氧化剂,骨转换,老年女性之间的BDNF指标在体力活动(PA)方面有所不同,以每日步数衡量。
方法:根据每天的步数评估62名72.1±5.4岁女性的PA水平,然后将参与者分为三组:第一组(n=18;每天<5,000步);第二组(n=22;每天5,000至9,999步);和第三组(n=22;每天≥10,000步)。在清晨从参与者中收集血液样本,并进行生化分析以确定促氧化剂-抗氧化剂平衡指标(SOD,CAT,GPx,GR,GSH,UA,MDA和TOS/TOC),骨代谢指标(Ca,25-OH维生素D,骨钙蛋白,CTX-I,和PTH),BDNF水平。
结果:各组SOD活性差异无统计学意义,CAT,GPx,和GR,但是它们的GSH(H=22.10,p<0.001)和UA(H=12.20,p=0.002)的浓度被证明与各组的每日PA显着相关。各组间MDA和TOS/TOC浓度差异不显著,这两个指标在I组中的值倾向于高于II和III组。在25-OH维生素D的浓度(H=24.21,p<0.001)之间建立了显着差异,骨钙蛋白(H=7.88,p=0.019),CTX-I(H=12.91,p=0.002),和BDNF(H=14.47,p=0.001),但不是Ca和PTH。
结论:GSH浓度显著升高,略低的氧化应激指标,显著高于BDNF水平,每天走超过5,000步的组中,适度更好的骨转换指标和吸收标志物表明,这种水平的PA可以促进成功的衰老。更多的研究是,然而,需要确认这一发现。
Given a lack of studies precisely indicating how many steps elderly people should take daily for their antioxidant defence, bone metabolism, and cognitive abilities to improve, our study set out to compare the selected antioxidant, prooxidant, bone turnover, and BDNF indicators between elderly women differing in physical activity (PA) measured by the daily number of steps.
The PA levels of 62 women aged 72.1 ± 5.4 years were assessed based on their daily number of steps and then were used to allocate the participants to three groups: group I (n = 18; <5,000 steps a day); group II (n = 22; from 5,000 to 9,999 steps a day); and group III (n = 22; ≥10,000 steps a day). Blood samples were collected from the participants in early morning hours and subjected to biochemical analysis for prooxidant-antioxidant balance indicators (SOD, CAT, GPx, GR, GSH, UA, MDA and TOS/TOC), bone metabolism indicators (Ca, 25-OH vitamin D, osteocalcin, CTX-I, and PTH), and BDNF levels.
The groups were not statistically significantly different in the activity of SOD, CAT, GPx, and GR, but their concentrations of GSH (H = 22.10, p < 0.001) and UA (H = 12.20, p = 0.002) proved to be significantly associated with the groups\' daily PA. The between-group differences in the concentrations of MDA and TOS/TOC were not significant, with both these indicators tending to take higher values in group I than in groups II and III. Significant differences between the groups were established for the concentrations of 25-OH vitamin D (H = 24.21, p < 0.001), osteocalcin (H = 7.88, p = 0.019), CTX-I (H = 12.91, p = 0.002), and BDNF (H = 14.47, p = 0.001), but not for Ca and PTH.
Significantly higher concentrations of GSH, slightly lower oxidative stress indicators, significantly higher BDNF levels, and moderately better bone turnover indicators and resorption markers in the group taking more than 5,000 steps a day suggest that this level of PA can promote successful aging. More research is, however, needed to confirm this finding.