■生物催化剂(酶)在催化各个行业的特定反应中起着至关重要的作用,经常提供环保和可持续的替代化学催化剂。然而,它们的催化活性容易变性。在这项研究中,我们发现了源自加工食品的新型蛋白质生物催化剂,包括脱脂牛奶,豆浆,奶酪,和干豆腐。这些食品催化剂具有很高的可用性,低成本,安全,和热稳定性。
■关注生理上有趣的辅酶吡咯并喹啉醌(PQQ),我们观察到,当PQQ以非常低的浓度存在时,与甘氨酸形成咪唑并吡咯喹啉(IPQ)的反应没有有效地进行。令人惊讶的是,在存在蛋白质食物的情况下,这一反应明显加快。值得注意的是,在IPQ衍生后的高效液相色谱(HPLC)中,脱脂乳提高了PQQ的检测限(降低了600倍)。牛奶似乎促进PQQ和各种氨基酸之间的反应,伯胺,和仲胺。进一步的研究表明,食品催化是通过非酶机理进行的。此外,核磁共振波谱表明,由于胺与胶体表面上的醌反应的能力,牛奶成分与氨基底物相互作用。
■这些实用的食品催化剂不仅有助于环境安全,而且在不同的科学领域具有重要意义。非酶蛋白催化剂在生物催化中的应用,有机合成,食品技术,分析化学,以及基础营养和进化研究。
UNASSIGNED: Biocatalysts (enzymes) play a crucial role in catalyzing specific reactions across various industries, often offering environmentally friendly and sustainable alternatives to chemical catalysts. However, their catalytic activities are susceptible to denaturation. In this study, we present the discovery of novel protein-based biocatalysts derived from processed foods, including skimmed milk, soy milk, cheese, and dried tofu. These food catalysts exhibit high availability, low cost, safety, and thermo-stability.
UNASSIGNED: Focusing on the physiologically intriguing coenzyme pyrroloquinoline quinone (PQQ), we observed that the reaction with glycine to form imidazolopyrroquinoline (IPQ) did not proceed efficiently when PQQ was present at very low concentrations. Surprisingly, in the presence of protein-based foods, this reaction was significantly accelerated. Notably, skimmed milk enhanced the PQQ detection limit (600 times lower) during high-performance liquid chromatography (HPLC) following IPQ derivatization. Milk appears to facilitate the reaction between PQQ and various amino acids, primary amines, and secondary amines. Further investigations revealed that food catalysis operates through a non-enzymatic mechanism. Additionally, nuclear magnetic resonance spectroscopy demonstrated that milk components interacted with amino substrates due to the ability of amines to react with quinones on colloidal surfaces.
UNASSIGNED: These practical food catalysts not only contribute to environmental safety but also hold significance across diverse scientific domains. Non-enzymatic protein catalysts find applications in biocatalysis, organic synthesis, food technology, analytical chemistry, and fundamental nutritional and evolutionary studies.