亚硝酸盐是淡水养殖环境中的主要环境污染物,这会对水生物种的生长产生负面影响。目前,我们知道亚硝酸盐进入甲壳类动物的主要途径是通过他们的g。在这项研究中,总共进行了96小时的急性亚硝酸盐应激(60毫克/升)实验,以及血清生化参数的影响,评估了红沼泽小龙虾的g氧化酶活性和氧化相关基因表达。暴露于亚硝酸盐0、6、12、24、48和96小时后,在每个时间点采集血淋巴和g样本.在血清中,急性亚硝酸盐应激显著增加谷氨酸-草酰乙酸转氨酶(GOT)和丙氨酸转氨酶(ALT)活性后6小时的暴露,暴露24小时和48小时后总蛋白(TP)和白蛋白(ALB)水平降低,分别。在the中,过氧化氢酶(CAT)的活性,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)在12h时增强到最大水平,24h和24h,分别。丙二醛(MDA)和过氧化脂质(LPO)含量在暴露12h和24h后显著增加,分别。此外,抗氧化相关基因的表达水平,包括hsp70,fer和mt,暴露6小时后,g中的表达显着上调。结果表明急性亚硝酸盐应激改变了血清生理状态,诱导氧化应激并对克氏疟原虫g细胞造成损伤。
Nitrite is the major environmental pollutant in the freshwater aquaculture environment, which has a negative impact on aquatic species growth. Currently, we know that the main way nitrite enters crustaceans is through their gills. In this study, a total of 96 h acute nitrite stress (60 mg/L) experiments were conducted, and the impact of the serum biochemical parameters, gill oxidase activity and oxidative-related gene expression of red swamp crayfish were evaluated. After exposure to nitrite for 0, 6, 12, 24, 48, and 96 h, hemolymph and gills samples were taken at each time point. In the serum, acute nitrite stress significantly increased glutamic-oxaloacetic transaminase (GOT) and alanine aminotransferase (ALT) activities after 6 h of exposure, decreased total protein (TP) and albumin (ALB) levels after 24 h and 48 h of exposure, respectively. In the gills, the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were enhanced to the maximum level at 12 h, 24 h and 24 h, respectively. The contents of malondialdehyde (MDA) and lipid peroxide (LPO) were increased significantly after 12 h and 24 h exposure, respectively. In addition, the expression levels of antioxidative-related genes, including hsp70, fer and mt, were significantly upregulated in the gills after 6 h of exposure. The results indicated that acute nitrite stress changed the serum physiological status, induced oxidative stress and caused damage to gill cells in P. clarkii.