目的:通过多样本实验建立口腔鳞状细胞癌侵袭下颌骨的动物模型,验证其稳定性,重复性,模型的致瘤性和下颌骨破坏率。
方法:将口腔鳞状细胞癌细胞悬液通过裸鼠咬肌前缘注射到下颌骨外侧,观察肿瘤形成过程。然后,解剖学,进行组织学和影像学检查以确定肿瘤是否已侵入下颌骨。通过比较多组各种鳞状细胞癌细胞(CAL27,HN6和HN30细胞)的肿瘤生长,比较了体重的变化和肿瘤形成的特征,总结经验,进一步验证稳定性,重复性,模型的肿瘤形成率和足弓损伤率。
结果:一旦建立了模型,就对随后的荷瘤裸鼠标本进行了验证。体外,肿瘤组织包裹在下颌骨的肿瘤承载侧,局部质地坚韧,对针刺无抵抗力。苏木素和伊红染色显示,鳞状细胞在水平面和矢状面上都浸润了下颌骨。显微计算机断层扫描结果表明,肿瘤侧的下颌骨表现出明显的侵蚀损伤。具有不同传代速率的细胞系显然具有不同的荷瘤生命周期。
结论:本研究成功建立了口腔鳞状细胞癌侵袭下颌骨的动物模型。该模子具有优越的生物稳固性,重复性,肿瘤发生率和下颌骨破坏率。
OBJECTIVE: To establish an animal model of oral squamous cell carcinoma invading the mandible through multi-sample experiments that verified the stability, repeatability, tumorigenicity and mandible destruction rate of the model.
METHODS: Oral squamous cell carcinoma cell suspension was injected into the outer side of the mandible through the anterior edge of the masseter muscle of naked mice to observe the tumourforming process. Then, the anatomical, histological and imaging examinations were carried out to determine whether the tumour had invaded the mandible. By comparing the tumour growth of multiple groups of various squamous cell carcinoma cells (CAL27, HN6 and HN30 cells), the changes in body weight and characteristics of tumour formation were compared, and the experience was summarised to further verify the stability, repeatability, tumour formation rate and arch damage rate of the model.
RESULTS: The subsequent specimens of tumour-bearing nude mice were validated once the model had been established. In vitro, tumour tissue wrapped around the mandible\'s tumour-bearing side, and the local texture was tough with no resistance to acupuncture. Haematoxylin and eosin staining revealed that squamous cells were infiltrating the mandible in both the horizontal and sagittal planes. Microcomputed tomography results showed that the mandible on the tumour-bearing side displayed obvious erosion damage. Cell lines with various passage rates clearly had diverse tumour-bearing life cycles.
CONCLUSIONS: This study successfully established an animal model of oral squamous cell carcinoma invasion of the mandible. The model has excellent biological stability, repeatability, tumorigenesis rate and mandible destruction rate.