To better understand the migration behavior of plastic fragments in the environment, development of rapid non-destructive methods for in-situ identification and characterization of plastic fragments is necessary. However, most of the studies had focused only on colored plastic fragments, ignoring colorless plastic fragments and the effects of different environmental media (backgrounds), thus underestimating their abundance. To address this issue, the present study used near-infrared spectroscopy to compare the identification of colored and colorless plastic fragments based on partial least squares-discriminant analysis (PLS-DA), extreme gradient boost, support vector machine and random forest classifier. The effects of polymer color, type, thickness, and background on the plastic fragments classification were evaluated. PLS-DA presented the best and most stable outcome, with higher robustness and lower misclassification rate. All models frequently misinterpreted colorless plastic fragments and its background when the fragment thickness was less than 0.1mm. A two-stage modeling method, which first distinguishes the plastic types and then identifies colorless plastic fragments that had been misclassified as background, was proposed. The method presented an accuracy higher than 99% in different backgrounds. In summary, this study developed a novel method for rapid and synchronous identification of colored and colorless plastic fragments under complex environmental backgrounds.

Antibiotic mycelia residues (AMRs) contain antibiotic residues. If AMRs are ingested in excess by livestock, it may cause health problems. To address the current problem of unknown pixel-scale adulteration concentration in NIR-HSI, this paper innovatively proposes a new spectral simulation method for the evaluation of AMRs in protein feeds. Four common protein feeds (soybean meal (SM), distillers dried grains with solubles (DDGS), cottonseed meal (CM), and nucleotide residue (NR)) and oxytetracycline residue (OR) were selected as study materials. The first step of the method is to simulate the spectra of pixels with different adulteration concentrations using a linear mixing model (LMM). Then, a pixel-scale OR quantitative model was developed based on the simulated pixel spectra combined with local PLS based on global PLS scores (LPLS-S) (which solves the problem of nonlinear distribution of the prediction results due to the 0%-100% content of the correction set). Finally, the model was used to quantitatively predict the OR content of each pixel in hyperspectral image. The average value of each pixel was calculated as the OR content of that sample. The implementation of this method can effectively overcome the inability of PLS-DA to achieve qualitative identification of OR in 2%-20% adulterated samples. In compared to the PLS model built by averaging the spectra over the region of interest, this method utilizes the precise information of each pixel, thereby enhancing the accuracy of the detection of adulterated samples. The results demonstrate that the combination of the method of simulated spectroscopy and LPLS-S provides a novel method for the detection and analysis of illegal feed additives by NIR-HSI.

Vigor is one of the important factors that affects rice yield and quality. Rapid and accurate detection of rice seed vigor is of great importance for rice production. In this study, near-infrared hyperspectral imaging technique and transfer learning were combined to detect rice seed vigor. Four varieties of artificial-aged rice seeds (Yongyou12, Yongyou1540, Suxiangjing100, and Longjingyou1212) were studied. Different convolutional neural network (CNN) models were built to detect the vigor of the rice seeds. Two transfer strategies, fine-tuning and MixStyle, were used to transfer knowledge among different rice varieties for vigor detection. The experimental results showed that the convolutional neural network model of Yongyou12 classified the vigor of Yongyou1540, Suxiangjing100, and Longjingyou1212 through MixStyle transfer knowledge, and the accuracy reached 90.00%, 80.33%, and 85.00% in validation sets, respectively, which was better or close to the initial modeling performances of each variety. MixStyle statistics are based on probabilistic mixed instance-level features of cross-source domain training samples. When training instances, new domains can be synthesized, which increases the domain diversity of the source domain, thereby improving the generalization ability of the trained model. This study would help rapid and accurate detection of a large varieties of crop seeds.

The limited nutritional information provided by external food representations has constrained the further development of food nutrition estimation. Near-infrared hyperspectral imaging (NIR-HSI) technology can capture food chemical characteristics directly related to nutrition and is widely used in food science. However, conventional data analysis methods may lack the capability of modeling complex nonlinear relations between spectral information and nutrition content. Therefore, we initiated this study to explore the feasibility of integrating deep learning with NIR-HSI for food nutrition estimation. Inspired by reinforcement learning, we proposed OptmWave, an approach that can perform modeling and wavelength selection simultaneously. It achieved the highest accuracy on our constructed scrambled eggs with tomatoes dataset, with a determination coefficient of 0.9913 and a root mean square error (RMSE) of 0.3548. The interpretability of our selection results was confirmed through spectral analysis, validating the feasibility of deep learning-based NIR-HSI in food nutrition estimation.

Ripening is the most crucial process step in cheese manufacturing and constitutes multiple biochemical alterations that describe the final cheese quality and its perceived sensory attributes. The assessment of the cheese-ripening process is challenging and requires the effective analysis of a multitude of biochemical changes occurring during the process. This study monitored the biochemical and sensory attribute changes of paraffin wax-covered long-ripening hard cheeses (n = 79) during ripening by collecting samples at different stages of ripening. Near-infrared hyperspectral (NIR-HS) imaging, together with free amino acid, chemical composition, and sensory attributes, was studied to monitor the biochemical changes during the ripening process. Orthogonal projection-based multivariate calibration methods were used to characterize ripening-related and orthogonal components as well as the distribution map of chemical components. The results approve the NIR-HS imaging as a rapid tool for monitoring cheese maturity during ripening. Moreover, the pixelwise evaluation of images shows the homogeneity of cheese maturation at different stages of ripening. Among the chemical compositions, fat content and moisture are the most important variables correlating to NIR-HS images during the ripening process.

Hyperspectral imaging combined with chemometric approaches is proven to be a powerful tool for the quality evaluation and control of fruits. In fruit defect-detection scenarios, developing an unsupervised anomaly detection framework is vital, as defect sample preparation is labor-intensive and time-consuming, especially for exploring potential defects. In this paper, a spectral-spatial, information-based, self-supervised anomaly detection (SSAD) approach is proposed. During training, an auxiliary classifier is proposed to identify the projection axes of principal component (PC) images that were transformed from the hyperspectral data cubes. In test time, the fully connected layer of the learned classifier was used as a \'spectral-spatial\' feature extractor, and the feature similarity metric was adopted as the score function for the downstream anomaly evaluation task. The proposed network was evaluated with two fruit data sets: a strawberry data set with bruised, infected, chilling-injured, and contaminated test samples and a blueberry data set with bruised, infected, chilling-injured, and wrinkled samples as anomalies. The results show that the SSAD yielded the best anomaly detection performance (AUC = 0.923 on average) over the baseline methods, and the visualization results further confirmed its advantage in extracting effective \'spectral-spatial\' latent representation. Moreover, the robustness of SSAD is verified with the data pollution experiment; it performed significantly better than the baselines when a portion of anomalous samples was involved in the training process.

Both nonthermal pretreatment and nondestructive analysis are effective technologies in improving drying processes. This study evaluated the effects of different pretreatment methods on the quality of beef dehydrated by microwave vacuum drying (MVD) and compared the MVD process performance comprising real-time moisture content (MC), MC loss, colour content, and shrinkage rate using different optical sensing methods including terahertz time-domain spectroscopy (THz-TDS) and near-infrared hyperspectral imaging (NIR-HSI). Results indicated that osmotic pretreatment improved the drying rate of MVD beef with lower changes in colour and shrinkage rate. Both THz-TDS-based and NIR-HSI-based on-site direct scanning and in-situ in-direct sensing showed accurate prediction results, with best R2p of 0.9646 and 0.9463 for MC and R2p of 0.9817 and 0.9563 for MC loss prediction, respectively. NIR-HSI visualisation of MC results showed that ultrasound pretreatment curbed but osmotic pretreatment promoted nonuniform distribution during MVD. This research should guide improving the industrial MVD drying process.

Root rot of Panax ginseng caused by Cylindrocarpon destructans, a soil-borne fungus is typically diagnosed by frequently checking the ginseng plants or by evaluating soil pathogens in a farm, which is a time- and cost-intensive process. Because this disease causes huge economic losses to ginseng farmers, it is important to develop reliable and non-destructive techniques for early disease detection. In this study, we developed a non-destructive method for the early detection of root rot. For this, we used crop phenotyping and analyzed biochemical information collected using the HSI technique. Soil infected with root rot was divided into sterilized and infected groups and seeded with 1-year-old ginseng plants. HSI data were collected four times during weeks 7-10 after sowing. The spectral data were analyzed and the main wavelengths were extracted using partial least squares discriminant analysis. The average model accuracy was 84% in the visible/near-infrared region (29 main wavelengths) and 95% in the short-wave infrared (19 main wavelengths). These results indicated that root rot caused a decrease in nutrient absorption, leading to a decline in photosynthetic activity and the levels of carotenoids, starch, and sucrose. Wavelengths related to phenolic compounds can also be utilized for the early prediction of root rot. The technique presented in this study can be used for the early and timely detection of root rot in ginseng in a non-destructive manner.

South African legislation regulates the classification/labelling and compositional specifications of raw beef patties, to combat processed meat fraud and to protect the consumer. A near-infrared hyperspectral imaging (NIR-HSI) system was investigated as an alternative authentication technique to the current destructive, time-consuming, labour-intensive and expensive methods. Eight hundred beef patties (ca. 100 g) were made and analysed to assess the potential of NIR-HSI to distinguish between the four patty categories (200 patties per category): premium \'ground patty\'; regular \'burger patty\'; \'value-burger/patty\' and the \'econo-burger\'/\'budget\'. Hyperspectral images were acquired with a HySpex SWIR-384 (short-wave infrared) imaging system using the Breeze® acquisition software, in the wavelength range of 952-2517 nm, after which the data was analysed using image analysis, multivariate techniques and machine learning algorithms. It was possible to distinguish between the four patty categories with accuracies ≥97%, indicating that NIR-HSI offers an accurate and reliable solution for the rapid identification and authentication of processed beef patties. Furthermore, this study has the potential of providing an alternative to the current authentication methods, thus contributing to the authenticity and fair-trade of processed meat products locally and internationally.

The evaluation of mutton adulteration faces new challenges because of mutton flavour essence, which achieves a similar flavour between the adulterant and mutton. Hence, methods for classifying and quantifying the adulterated mutton under the effect of mutton flavour essence, based on near-infrared hyperspectral imaging (NIR-HSI, 1000-2500 nm) combined with machine learning (ML) and sparrow search algorithm (SSA), were proposed in this study. After spectral preprocessing via first derivative combined with multiple scattering correction (1D + MSC), classification and quantification models were established using back propagation neural network (BP), extreme learning machine (ELM) and support vector machine/regression (SVM/SVR). SSA was further used to explore the global optimal parameters of these models. Results showed that the performance of models improves after optimisation via the SSA. SSA-SVM achieved the optimal discrimination result, with an accuracy of 99.79% in the prediction set; SSA-SVR achieved the optimal prediction result, with an RP2 of 0.9304 and an RMSEP of 0.0458 g·g-1. Hence, NIR-HSI combined with ML and SSA is feasible for classification and quantification of mutton adulteration under the effect of mutton flavour essence. This study can provide a theoretical and practical reference for the evaluation and supervision of food quality under complex conditions.