目的:本研究旨在探讨手术干预对阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患儿外周血T淋巴细胞亚群和自然杀伤(NK)细胞活性的影响。
方法:共36名OSAHS儿童,32名扁桃体肥大儿童,30名健康儿童入组.收集临床数据和多导睡眠图(PSG)结果。外周血样本进行T淋巴细胞亚群分析,NK细胞,和细胞因子水平,包括Th1(IFN-γ,IL-2,TNF-α),Th2(IL-4,IL-10),Th17(IL-17)。
结果:在基线时,与健康儿童相比,OSAHS儿童表现出更低的LSaO2水平和更高的AHI值。他们还显示CD3+T细胞的百分比下降,CD4+T细胞,NK细胞,和升高的CD8+T细胞和CD4+/CD8+比率。IFN-γ水平,IL-2,TNF-α,IL-4和IL-17在OSAHS儿童中显著降低。术后改善观察到LSaO2,AHI,和免疫标记在3个月和6个月。Pearson的相关分析显示LSaO2、AHI、基线和术后6个月时的外周血免疫参数。
结论:手术干预对儿童OSAHS患者外周血T淋巴细胞亚群和NK细胞活性有影响。早期干预和免疫功能的监测对于患病儿童的恢复和健康发展至关重要。
OBJECTIVE: This study aimed to investigate the impact of surgical intervention on peripheral blood T lymphocyte subsets and natural killer (NK) cell activity in pediatric patients with obstructive sleep apnea hypopnea syndrome (OSAHS).
METHODS: A total of 36 OSAHS children, 32 children with tonsillar hypertrophy, and 30 healthy children were enrolled. Clinical data and polysomnography (PSG) results were collected. Peripheral blood samples were analyzed for T lymphocyte subsets, NK cells, and cytokine levels including Th1 (IFN-γ, IL-2, TNF-α), Th2 (IL-4, IL-10), and Th17 (IL-17).
RESULTS: At baseline, OSAHS children exhibited lower LSaO2 levels and higher AHI values compared to healthy children. They also showed decreased percentages of CD3 + T cells, CD4 + T cells, NK cells, and elevated CD8 + T cells and CD4+/CD8 + ratio. Levels of IFN-γ, IL-2, TNF-α, IL-4, and IL-17 were significantly lower in OSAHS children. Post-surgery improvements were observed in LSaO2, AHI, and immune markers at 3 months and 6 months. Pearson\'s correlation analysis revealed significant associations between LSaO2, AHI, and peripheral blood immune parameters at baseline and 6 months post-surgery.
CONCLUSIONS: Surgical intervention in pediatric OSAHS influences peripheral blood T lymphocyte subsets and NK cell activity. Early intervention and monitoring of immune function are crucial for the recovery and healthy development of affected children.