■研究表明,微塑料(MPs)和纳米塑料(NPs)可能在人体内积聚,对人类健康构成潜在威胁。本研究的目的是全面,彻底地评估不同粒径的MPs/NPs的生物分布和毒性。
■本研究的目的是研究不同尺寸(50nm,100nm,和500nm)。给BALB/c小鼠以1mg/kgBW或10mg/kgBW的剂量给予100μLPS50,PS100和PS500,分别,每天灌胃一次。连续治疗28天后,通过冷冻切片荧光显微镜和荧光酶标仪分析确定不同大小的PSMPs/NP的生物分布,以及不同大小的PSMPs/NPs对组织病理学的后续影响,还评估了血液学和血液生物化学。
■结果表明,三种不同大小的PSMPs/NPs分布在小鼠的器官中,主要在肝脏,脾,脾和肠。同时,颗粒尺寸越小,它们在体内积累的越多,更容易穿透组织。在整个观察期间,未观察到异常行为和体重变化。H&E染色结果显示,低剂量染毒组主要脏器未见严重组织病理学异常,while.暴露三种大小的PSMPs/NP可能会导致与心脏相关的血液学参数或生化参数的某些变化,肝脏,和肾功能;同时,有大小和剂量依赖性.
■随着粒径的减小和塑料颗粒浓度的增加,塑料颗粒在小鼠体内的生物分布和毒性更加明显。与国会议员相比,NP更容易进入组织并在肝脏中产生变化,肾,和心脏功能。因此,应重视NPs的毒性。
UNASSIGNED: Studies have shown that microplastics (MPs) and nanoplastics (NPs) could accumulate in the human body and pose a potential threat to human health. The purpose of this study is to evaluate the biodistribution and toxicity of MPs/NPs with different particle sizes comprehensively and thoroughly.
UNASSIGNED: The purpose of this study was to investigate the biodistribution and in vivo toxicity of polystyrene (PS) MPs/NPs with different sizes (50 nm, 100 nm, and 500 nm). The BALB/c mice were given 100 μL of PS50, PS100 and PS500 at the dosage of 1 mg/kg BW or 10 mg/kg BW, respectively, by gavage once a day. After 28 consecutive days of treatment, the biodistribution of differently sized PS MPs/NPs was determined through cryosection fluorescence microscopy and fluorescent microplate reader analysis, and the subsequent effects of differently sized PS MPs/NPs on histopathology, hematology and blood biochemistry were also evaluated.
UNASSIGNED: The results showed that the three different sizes of PS MPs/NPs were distributed in the organs of mice, mainly in the liver, spleen, and intestine. At the same time, the smaller the particle size, the more they accumulate in the body and more easily penetrate the tissue. During the whole observation period, no abnormal behavior and weight change were observed. The results of H&E staining showed that no severe histopathological abnormalities were observed in the main organs in the low-dose exposure group, while. Exposure of three sizes of PS MPs/NPs could cause some changes in hematological parameters or biochemical parameters related to heart, liver, and kidney function; meanwhile, there were size- and dose-dependencies.
UNASSIGNED: The biological distribution and toxicity of plastic particles in mice were more obvious with the decrease of particle size and the increase of concentration of plastic particles. Compared with MPs, NPs were easier to enter the tissues and produce changes in liver, kidney, and heart functions. Therefore, more attention should be paid to the toxicity of NPs.