肝细胞癌(HCC)是一种常见的起源于肝细胞的恶性肿瘤。其特点是复杂的发病机制和有限的治疗选择,如手术,化疗,和移植。顺铂,一种有效的化疗药物,破坏癌细胞DNA,但受到副作用和需要控制持续释放以优化功效的阻碍。金属有机框架(MOFs)已成为有前途的纳米载体,用于精确的局部药物输送。减少所需剂量并减轻化疗药物的副作用,从而为肝细胞癌(HCC)的治疗提供了一个潜在的途径。在这项研究中,矩形通道MOF(RumgayH,FerlayJ,MartelC,乔治·D,IbrahimAS,郑R,魏W,LemmensVEPP,SoerjomataramI(2022)全球,按亚型划分的原发性肝癌的地区和国家负担。使用配体L作为与Cu(II)和I(I)配位的有机接头合成了EurJCancer161:108-118)载体。对MOF的结构和荧光性质进行了表征。此外,为了增强底物的生物相容性,通过将聚乳酸(PLA)与用于顺铂负载的1掺入来制备复合载体材料。评价PLA-1@顺铂对肝癌的抑制作用,HepG-2和Huh-7HCC细胞系用不同浓度的药物处理48小时,并评估它们的细胞活力。结果显示HepG-2和Huh-7细胞的细胞活力显著剂量依赖性降低。探讨PLA-1@顺铂对肝癌的潜在抑制机制,测定治疗后HepG-2和Huh-7细胞中GADD45A和NACC1的mRNA水平。GADD45A表达,最初在HCC细胞中含量较低,药物治疗后显著上调,而NACC1通常在HCC中高表达,随着PLA-1@顺铂浓度的增加,mRNA水平显着降低。这些发现表明PLA-1@顺铂有效上调GADD45A表达并下调NACC1表达。总的来说,开发的负载顺铂的纳米颗粒系统通过减少化疗副作用和提高药物疗效,有望用于HCC治疗。
Hepatocellular carcinoma (HCC) is a common malignant tumor originating from liver cells, characterized by complex pathogenesis and limited treatment options such as surgery, chemotherapy, and transplantation. Cisplatin, an effective chemotherapeutic agent, disrupts cancer cell DNA but is hindered by side effects and the need for controlled sustained release to optimize efficacy. Metal-organic frameworks (MOFs) have emerged as promising nanocarriers for precise local drug delivery, reducing required doses and mitigating side effects of chemotherapeutic drugs, thus offering a potential avenue for hepatocellular carcinoma (HCC) treatment. In this research, a rectangular channel MOF (Rumgay H, Ferlay J, Martel C, Georges D, Ibrahim AS, Zheng R, Wei W, Lemmens VEPP, Soerjomataram I (2022) Global, regional and national burden of primary liver cancer by subtype. Eur J Cancer 161:108-118) carrier was synthesized using ligand L as the organic linker coordinated with Cu(II) and I(I). The MOF\'s structure and fluorescence properties were characterized. Additionally, to enhance substrate biocompatibility, composite carrier materials were prepared by incorporating polylactic acid (PLA) with 1, utilized for cisplatin loading. To evaluate the inhibitory effect of PLA-1@cisplatin on HCC, HepG-2 and Huh-7 HCC cell lines were treated with varying concentrations of the drug for 48 h, and their cell viability was assessed. The results demonstrated a significant dose-dependent reduction in cell viability of both HepG-2 and Huh-7 cells. To explore the potential inhibitory mechanism of PLA-1@cisplatin on HCC, the mRNA levels of GADD45A and NACC1 in HepG-2 and Huh-7 cells post-treatment were measured. GADD45A expression, initially low in HCC cells, was significantly upregulated after drug treatment, while NACC1, typically highly expressed in HCC, showed a significant decrease in mRNA levels with increasing concentrations of PLA-1@cisplatin. These findings indicate that PLA-1@cisplatin effectively upregulates GADD45A expression and downregulates NACC1 expression. Overall, the developed cisplatin-loaded nanoparticle system holds promise for HCC treatment by reducing chemotherapy side effects and enhancing drug efficacy.