硫化氢(H2S)作为一种新型的内源性气体信号分子,加入一氧化氮(NO)和一氧化碳(CO)的行列。最近的研究强调了它参与各种生理过程,例如促进根器官发生,调节气孔运动和光合作用,促进植物生长,发展,和抗压力。烟草,对农民经济收入至关重要的重要经济作物,严重依赖根系发育来影响叶片生长,抗病性,化学成分,和产量。尽管它很重要,仍然缺乏研究H2S在促进烟草生长中的作用的研究。本研究将烟草幼苗暴露于不同浓度的NaHS(外源H2S供体)-0、200、400、600和800mg/L。结果表明,NaHS浓度与根长呈正相关,湿重,根系活动,和抗氧化酶活性(CAT,SOD,和POD)在烟草根中。转录组学和代谢组学分析显示,用600mg/LNaHS处理显著影响162个关键基因,44种关键酶,和烟草幼苗中的两个代谢途径(油菜素类固醇合成和天冬氨酸生物合成)。添加外源NaHS不仅促进了烟草根系发育,而且可能减少了农药的使用。为更可持续的生态环境做出贡献。总的来说,本研究揭示了烟草根系对NaHS反应的主要代谢途径,为植物根系发育的未来研究提供新的遗传见解。
Hydrogen sulfide (H2S) has emerged as a novel endogenous gas signaling molecule, joining the ranks of nitric oxide (NO) and carbon monoxide (CO). Recent research has highlighted its involvement in various physiological processes, such as promoting root organogenesis, regulating stomatal movement and photosynthesis, and enhancing plant growth, development, and stress resistance. Tobacco, a significant cash crop crucial for farmers\' economic income, relies heavily on root development to affect leaf growth, disease resistance, chemical composition, and yield. Despite its importance, there remains a scarcity of studies investigating the role of H2S in promoting tobacco growth. This study exposed tobacco seedlings to different concentrations of
NaHS (an exogenous H2S donor) - 0, 200, 400, 600, and 800 mg/L. Results indicated a positive correlation between
NaHS concentration and root length, wet weight, root activity, and antioxidant enzymatic activities (CAT, SOD, and POD) in tobacco roots. Transcriptomic and metabolomic analyses revealed that treatment with 600 mg/L
NaHS significantly effected 162 key genes, 44 key enzymes, and two metabolic pathways (brassinosteroid synthesis and aspartate biosynthesis) in tobacco seedlings. The addition of exogenous
NaHS not only promoted tobacco root development but also potentially reduced pesticide usage, contributing to a more sustainable ecological environment. Overall, this study sheds light on the primary metabolic pathways involved in tobacco root response to
NaHS, offering new genetic insights for future investigations into plant root development.