2,4-二硝基苯胺(2,4-D),一种广泛使用的染料中间体,是典型的污染物之一,其潜在的健康风险和毒性在很大程度上仍然未知。探讨其亚慢性口服毒性,Wistar大鼠(雄性和雌性数量相等)用作测试动物,并进行了90天的口服给药实验,分为对照组,低剂量组(0.055mg/kg),中剂量组(0.22mg/kg),中高剂量组(0.89mg/kg),高剂量组(3.56mg/kg)。体重数据,临床表现,记录每只受试大鼠在给药90天内的药物反应;收集4次早晨尿液样本,检测8项尿液指标;收集血液样本,检测19项血液学指标和16项生化指标;收集组织样本进行病理分析;确定了未观察到的不良反应水平(NOAEL),和基准剂量方法被用来支持这一确定,并提供相应剂量的统计估计。结果表明,2,4-二硝基苯胺的慢性毒性表现出一定的性别差异,用眼睛,肝脏,肾脏是毒性的主要潜在靶器官。此外,2,4-二硝基苯胺的亚慢性口服NOAEL测定为0.22mg/kg体重(男性为0.22mg/kg,女性为0.89mg/kg),初步计算人体安全接触限值为0.136mg/kg。研究结果极大地丰富了2,4-二硝基苯胺的安全性评价数据,为制定知情的安全法规和公共卫生预防措施奠定了坚实的科学基础。
2,4-dinitroaniline (2,4-D), a widely used dye intermediate, is one of the typical pollutants, and its potential health risks and toxicity are still largely unknown. To explore its subchronic oral toxicity, Wistar rats (equal numbers of males and females) were used as test animals, and a 90-day oral dosing experiment was conducted, divided into control group, low-dose group (0.055 mg/kg), medium-dose group (0.22 mg/kg), medium-high dose group (0.89 mg/kg), and high-dose group (3.56 mg/kg). The body weight data, clinical appearance, and drug reactions of each test rat within 90 days of dosing were recorded; morning urine samples were collected four times to test for eight urinary indicators; blood samples were collected to test for nineteen hematological indicators and sixteen biochemical indicators; tissue samples were collected for pathological analysis; moreover, the no-observed-adverse-effect level (
NOAEL) was determined, and the benchmark dose method was used to support this determination and provide a statistical estimate of the dose corresponding. The results indicated that the chronic toxicity of 2,4-dinitroaniline showed certain gender differences, with the eyes, liver, and kidneys being the main potential target organs of toxicity. Moreover, the subchronic oral
NOAEL for 2,4-dinitroaniline was determined to be 0.22 mg/kg body weight (0.22 mg/kg for males and 0.89 mg/kg for females), and a preliminary calculation of the safe exposure limit for human was 0.136 mg/kg. The research results greatly enriched the safety evaluation data of 2,4-dinitroaniline, contributing to a robust scientific foundation for the development of informed safety regulations and public health precautions.