■许多市售的近红外(NIR)荧光成像系统缺乏用于实时可量化荧光数据的算法。用于临床评估和事后分析的工作流程的创建可以为临床研究人员提供用于术中荧光定量以改善客观结果测量的方法。
■采用评分系统和经过验证的图像分析来确定手术标本中和术后的荧光量和强度。
■在肿瘤周围注射吲哚菁绿(ICG)后,在淋巴结摘除过程中获得了犬癌患者的淋巴结。首先,评估了表面荧光的半定量评估。分析用NIR镜获得的图像以确定荧光阈值并测量荧光量和强度。
■后荧光定量(色调的阈值=165-180,强度=30-255)显示出与半定量评分的强烈一致性(k=0.9734,p<0.0001)。阈值为35-255或45-255的荧光强度是荧光的重要预测因子,并且具有高灵敏度和特异性(p<0.05)。荧光强度和定量具有很强的相关性(p<0.001)。
■通过图像分析对半定量评分系统的验证提供了一种客观原位观察组织荧光的方法。对ICG荧光强度的阈值化的利用允许在未构建到成像系统中时对荧光进行事后量化。
UNASSIGNED: Many commercially available near-infrared (NIR) fluorescence imaging systems lack algorithms for real-time quantifiable fluorescence data. Creation of a workflow for clinical assessment and post hoc analysis may provide clinical researchers with a method for intraoperative fluorescence quantification to improve objective outcome measures.
UNASSIGNED: Scoring systems and verified image analysis are employed to determine the amount and intensity of fluorescence within surgical specimens both intra and postoperatively.
UNASSIGNED: Lymph nodes from canine cancer patients were obtained during lymph node extirpation following peritumoral injection of indocyanine green (ICG). First, a semi-quantitative assessment of surface fluorescence was evaluated. Images obtained with a NIR exoscope were analysed to determine fluorescence thresholds and measure fluorescence amount and intensity.
UNASSIGNED: Post hoc fluorescence quantification (threshold of Hue = 165-180, Intensity = 30-255) displayed strong agreement with semi-quantitative scoring (k = 0.9734, p < 0.0001). Fluorescence intensity with either threshold of 35-255 or 45-255 were significant predictors of fluorescence and had high sensitivity and specificity (p < 0.05). Fluorescence intensity and quantification had a strong association (p < 0.001).
UNASSIGNED: The validation of the semi-quantitative scoring system by image analysis provides a method for objective in situ observation of tissue fluorescence. The utilization of thresholding for ICG fluorescence intensity allows post hoc quantification of fluorescence when not built into the imaging system.