目的:本研究的目的是评估体外用初榨椰子油(VCO)处理并水解初榨椰子油(HVCO)的NIH3T3细胞中的蛋白质表达。
方法:本研究中使用的椰子油是初榨椰子油(VCO)和经米黑根虫水解的VCO(HVCO)。将NIH3T3细胞(5x105个细胞/孔)接种在9个孔中并孵育过夜,然后分成三组。每组由三个孔组成。第一组没有治疗,第二组增加了VCO,第三组加入HVCO,然后孵育过夜。每组各1孔加入MMP-9、PDGF-BB,和TGF-β1并孵育1小时。最后,MMP-9、PDGF-BB、免疫细胞化学法检测TGF-β1。
结果:研究结果表明,VCO和HVCO增加MMP-9,PDGF-BB的蛋白表达,和TGF-β1。VCO处理的MMP-9表达百分比从2.89±0.07增加到28.16±0.34,PDGF-BB从28.11±0.13增加到48.53±0.49,TGF-β1从4.19±0.08增加到18.41±0.54。HVCO处理的MMP-9表达百分比从2.89±0.07增加到55.40±0.94,PDGF-BB从28.11±0.13增加到61.65±0.42,TGF-β1从4.19±0.08增加到36.35±0.67。
结论:VCO和HVCO增加MMP-9、PDGF-BB的表达,在NIH3T3细胞中danTGF-β1,椰子油在伤口愈合过程中活跃。HVCO比VCO更活跃。
OBJECTIVE: The objective of the study was to evaluate protein expression in NIH 3T3 cells that are treated with virgin coconut oil (VCO) and hydrolysed of virgin coconut oil (HVCO) in vitro.
METHODS: Coconut oil used in this study was virgin coconut oil (VCO) and VCO hydrolysed by Rhizomucor miehei (HVCO). NIH 3T3 cells (5x105 cells/well) were seeded in nine wells and incubated for overnight, then divided into three groups. Each group consisted of three wells. Group one without treatment, group two added VCO, and group three added HVCO and then incubated for overnight. One well in each group was added MMP-9, PDGF-BB, and TGF-β1 and incubated one hour. Finally, expressions of MMP-9, PDGF-BB, and TGF-β1 were detected using immunocytochemistry method.
RESULTS: The results of the study showed that VCO and HVCO increased protein expressions of MMP-9, PDGF-BB, and TGF-β1. Percentage of MMP-9 expressions treated by VCO increased from 2.89 ± 0.07 to 28.16 ± 0.34, PDGF-BB from 28.11 ± 0.13 to 48.53 ± 0.49, and TGF-β1 from 4.19 ± 0.08 to 18.41 ± 0.54. Percentage of MMP-9 expressions treated by HVCO increased from 2.89 ± 0.07 to 55.40 ± 0.94, PDGF-BB from 28.11 ± 0.13 to 61.65 ± 0.42, and TGF-β1 from 4.19 ± 0.08 to 36.35 ± 0.67.
CONCLUSIONS: VCO and HVCO increase the expression of MMP-9, PDGF-BB, dan TGF-β1 in NIH3T3 cells and therefore, coconut oil active in the wound healing process. HVCO is more than active than VCO.