背景:义齿口腔炎(DS)是一种炎症性疾病,会影响义齿下方的粘膜表面,并经常引起口腔粘膜刺激,不适,改变了味觉,阻止人们摄入足够的营养。DS的主要原因之一是真菌白色念珠菌的过度生长(C.albicans).念珠菌感染的一种可能的替代疗法被认为是激光治疗。这项研究的目的是评估不同波长的激光如何影响白色念珠菌的生长和致病特性。
方法:在制备过程中使用浓度为106活细胞/ml的白色念珠菌。从标本中创建四组。对照组的培养在没有干预的情况下完成。其他3组以1W的功率接收激光辐射60秒。第2组和第3组用二极管激光分别在940nm和980nm的波长下照射。第4组用波长为1064nm的Nd-YAG激光照射。比浊生长被定义为真菌生长的光密度的变化。这些措施是在三个不同的时间进行的:基线,48小时,72小时
结果:在两组二极管激光器中,白色念珠菌的生长在基线时没有显着差异,在48和72小时后,使用1W的功率和60秒的持续时间。Nd-YAG组显示光密度在48小时后显著增加,然后在72小时后显著降低。对照组的光密度值在不同时间段在对照组和二极管研究组之间没有显着差异。然而,Nd:YAG组与对照组和2个半导体激光组相比,差异有统计学意义。
结论:不同的激光参数对白色念珠菌的生长和致病特性有不同的影响。在连续模式下使用波长940和980nm的二极管激光治疗,功率为1W,持续时间为60秒,可导致白色念珠菌增殖,而不是破坏它们。Nd:YAG激光器,用于脉冲模式,功率为1W,持续时间为60秒,可用于破坏白色念珠菌,因此,可作为义齿口炎的有效治疗方法。
BACKGROUND: Denture stomatitis (DS) is an inflammatory disorder that affects the mucosal surface underneath the dentures and frequently causes oral mucosal irritation, discomfort, and altered taste perception, which prevents people from consuming enough nutrients. One of the main causes of DS is an overgrowth of the fungus Candida albicans (C. albicans). A possible alternative treatment for Candida infections is thought to be laser therapy. The aim of this study was to evaluate how different wavelengths of laser would affect growth and pathogenic properties of Candida albicans.
METHODS: A concentration of 106 viable cells/ml of Candida albicans were used in the preparation process. Four groups were created from the specimens. Culturing of the control group was completed with no intervention. The other 3 groups received laser radiation for 60 seconds at a power of 1W. The 2nd and 3rd groups were irradiated with diode laser at a wavelength of 940 nm and 980 nm respectively. The 4th group was irradiated with Nd-YAG laser at a wavelength of 1064 nm. Turbidimetric growth was defined as variations in the optical density of fungal growth. These measures were made at three different times: baseline, 48 hours, and 72 hours.
RESULTS: In both groups of diode laser, the growth of Candida albicans showed no remarkable differences at baseline, after 48 and 72 hours using a power of 1 W and duration of 60 seconds. The Nd-YAG group showed significant increase in optical density after 48 hrs then significant decrease after 72 hrs. The optical density values in the control group showed no notable difference between the control and diode study groups at different time periods. However, the Nd:YAG group showed a statistically significant difference compared to the control and the 2 diode laser groups.
CONCLUSIONS: Different laser parameters have a different effect on growth and pathogenic properties of Candida albicans. Diode laser therapy with wavelengths 940 and 980 nm used in continuous mode with power of 1 W for duration of 60 seconds can result in proliferation of Candida albicans instead of destroying them. Nd:YAG laser, used in pulsed mode, with power of 1 W for a duration of 60 seconds can be used to destroy Candida albicans and therefore, can be used as an effective treatment for denture stomatitis.