■肝细胞癌(HCC)是一种常见的腹部肿瘤,生存结果较差。尽管越来越多的证据表明N7-甲基鸟苷(m7G)与肿瘤预后密切相关,发展,和免疫反应,很少有研究关注这个话题。
■通过Lasso回归分析构建了新的m7G风险特征。通过一系列生存分析评估了其预后价值,并在ICGC-LIRI中进行了测试。GSE14520和GSE116174队列。CIBERSORT,ssGSEA,和ESTIMATE方法用于探讨m7G风险评分对肿瘤免疫微环境(TIM)的影响。GSEA方法用于评估m7G风险评分对糖酵解的影响,铁性凋亡,和焦亡。人蛋白质图谱(HPA)数据库用于阐明五个m7G标记基因的组织学表达水平。通过qPCR证实NCBP2在肝细胞癌(HC)细胞中的生物学功能,CCK8和transwell测定。
■五个m7G调控基因包含了新的风险特征。m7G风险评分被确定为HCC的独立预后因素,并且可以增加传统预后模型的决策益处。此外,我们建立了包含临床分期和m7G风险评分的列线图来预测HCC患者的生存率.在ICGC和GSE116174队列中成功验证了m7G模型的预后价值。此外,高m7G风险导致CD8+T细胞浸润水平降低,而它增加了Tregs和巨噬细胞的浸润水平。发现糖酵解和焦亡过程在具有高m7G风险的HCC患者中富集。最后,NCBP2的过表达可以促进细胞增殖,迁移,和HC细胞的入侵。
■m7G风险评分与预后密切相关,抗肿瘤免疫过程,糖酵解,和肝癌的恶性进展。NCBP2具有致癌能力,显示出作为一种新的治疗目标的希望。
UNASSIGNED: Hepatocellular carcinoma (HCC) is a common abdominal cancer with poor survival outcomes. Although there is growing evidence that N7-methylguanosine (m7G) is closely associated with tumor prognosis, development, and immune response, few studies focus on this topic.
UNASSIGNED: The novel m7G risk signature was constructed through the Lasso regression analysis. Its prognostic value was evaluated through a series of survival analyses and was tested in ICGC-LIRI, GSE14520, and GSE116174 cohorts. CIBERSORT, ssGSEA, and ESTIMATE methods were applied to explore the effects of the m7G risk score on tumor immune microenvironment (TIM). The GSEA method was used to evaluate the impacts of the m7G risk score on glycolysis, ferroptosis, and pyroptosis. The human protein atlas (HPA) database was used to clarify the histological expression levels of five m7G signature genes. The biofunctions of
NCBP2 in hepatocellular cancer (HC) cells were confirmed through qPCR, CCK8, and transwell assays.
UNASSIGNED: Five m7G regulatory genes comprised the novel risk signature. The m7G risk score was identified as an independent prognostic factor of HCC and could increase the decision-making benefit of traditional prognostic models. Besides, we established a nomogram containing the clinical stage and m7G risk score to predict the survival rates of HCC patients. The prognostic value of the m7G model was successfully validated in ICGC and GSE116174 cohorts. Moreover, high m7G risk led to a decreased infiltration level of CD8+ T cells, whereas it increased the infiltration levels of Tregs and macrophages. The glycolysis and pyroptosis processes were found to be enriched in the HCC patients with high m7G risk. Finally, overexpression of
NCBP2 could promote the proliferation, migration, and invasion of HC cells.
UNASSIGNED: The m7G risk score was closely related to the prognosis, antitumor immune process, glycolysis, and malignant progression of HCC.
NCBP2 has pro-oncogenic abilities, showing promise as a novel treatment target.