呼吸爆发氧化酶同源物(RBOHs),也称为NADPH氧化酶,对植物中ROS的产生有很大的贡献,以及其他主要来源,如叶绿体中的光合作用和电子传输。研究表明,植物RBOHs在植物逆境响应和电子传递中起着积极的作用。然而,尚未对番茄中SlRBOH基因家族的系统发育分析和表征进行系统研究。这项研究使用全基因组搜索方法鉴定了番茄基因组中的11个SlRBOH基因。物理化学性质,染色体定位,亚细胞定位,二级结构,保守的图案,基因结构,系统发育学,共线关系,顺式作用元素,进化选择的压力,组织表达,并分析了外源植物激素(ABA和MeJA)和不同非生物胁迫下的表达模式。我们发现SlRBOHs分布在七个染色体上,共线性反映了它们与拟南芥和水稻中相应基因的进化关系。此外,所有SlRBOH成员都有5个保守结构域和10个保守基序,并具有相似的基因结构。此外,进化选择压力分析的结果表明,SlRBOH家族成员主要通过纯化选择进化,使它们结构更稳定。顺式作用元素分析表明,SlRBOHs对光有反应,激素,和非生物胁迫。组织表达分析表明,SlRBOH家族成员在番茄各组织中均有不同程度的表达,在根中发现了大多数具有最强表达的SlRBOHs。此外,通过ABA改变番茄SlRBOH基因的表达,MeJA,暗期延长,NaCl,PEG,UV,冷,热,和H2O2处理。具体来说,SlRBOH4在NaCl下高表达,PEG,热,和紫外线处理,而SlRBOH2在冷胁迫下高表达。这些结果为进一步研究SlRBOHs在番茄中的功能奠定了基础。
Respiratory burst oxidase homologs (RBOHs), also known as NADPH oxidases, contribute significantly to the production of ROS in plants, alongside other major sources such as photosynthesis and electron transport in chloroplasts. It has been shown that plant RBOHs play an active role in plant adversity response and electron transport. However, the phylogenetic analysis and characterization of the SlRBOH gene family in tomatoes have not been systematically studied. This study identified 11 SlRBOH genes in the tomato genome using a genome-wide search approach. The physicochemical properties, chromosomal localization, subcellular localization, secondary structure, conserved motifs, gene structure, phylogenetics, collinear relationships, cis-acting elements, evolutionary selection pressures, tissue expressions, and expression patterns under exogenous phytohormones (ABA and MeJA) and different abiotic stresses were also analyzed. We found that the SlRBOHs are distributed across seven chromosomes, collinearity reflecting their evolutionary relationships with corresponding genes in Arabidopsis thaliana and rice. Additionally, all the SlRBOH members have five conserved domains and 10 conserved motifs and have similar gene structures. In addition, the results of an evolutionary selection pressure analysis showed that SlRBOH family members evolved mainly by purifying selection, making them more structurally stable. Cis-acting element analyses showed that SlRBOHs were responsive to light, hormone, and abiotic stresses. Tissue expression analysis showed that SlRBOH family members were expressed in all tissues of tomato to varying degrees, and most of the SlRBOHs with the strongest expression were found in the roots. In addition, the expressions of tomato SlRBOH genes were changed by ABA, MeJA, dark period extension, NaCl, PEG, UV, cold, heat, and H2O2 treatments. Specifically, SlRBOH4 was highly expressed under NaCl, PEG, heat, and UV treatments, while SlRBOH2 was highly expressed under cold stress. These results provide a basis for further studies on the function of SlRBOHs in tomato.