未经证实:产生blaNDM-1的鲍曼不动杆菌(BP-AB)仍然是医院感染的关键问题,由于其抗性由生物膜形成和毒力因子介导。没有研究证实了在抑制与BP-AB的生物膜相关的生物膜形成和毒力方面的效果。
UNASSIGNED:测试的甲酚浓度为野生型(A),50μg/mL(B),100μg/mL(C),200μg/mL(D),250μg/mL(E),和300μg/mL(F)。
UNASSIGNED:在D,E,F组比A组多,B,C组。Myrtenol可显着减少玻璃管中气液界面环的形成。如结晶紫染色所示,它还有效抑制了BP-AB菌株在聚苯乙烯表面的附着。显微镜检查显示,分散的BP-AB菌株的生物膜形成显着减少。共聚焦激光扫描显微镜分析显示生物膜的生物量显著减少,覆盖表面积,和厚度。扫描电子显微镜分析显示盖玻片表面上的BP-AB聚集体明显减少。在CompStat分析中,生物膜的生物量,最大厚度,和表面体积比显著降低。qPCR分析显示bfmR的显著下调,bap,csuA/B,和ompA表达式,与生物膜的生物量呈正相关,最大厚度,BP-AB菌株的表面体积比。Myrtenol可显著提高BP-AB对阿米卡星的敏感性,哌拉西林/他唑巴坦,头孢哌酮/舒巴坦,还有头孢他啶.
UNASSIGNED:Myrtenol通过抑制bfmR的表达来减弱BP-AB生物膜的形成和毒力,bap,csuA/B,ompa.
UNASSIGNED: blaNDM-1-producing Acinetobacter baumannii (BP-AB) remains a critical problem in nosocomial infections, because of its resistance mediated by the biofilm formation and virulence factors. No studies have confirmed
myrtenol\'s efficacy in inhibiting the biofilm formation and virulence associated with biofilm of BP-AB.
UNASSIGNED: The tested concentrations of
myrtenol were wild type (A), 50 μg/mL (B), 100 μg/mL (C), 200 μg/mL (D), 250 μg/mL (E), and 300 μg/mL (F).
UNASSIGNED: The BP-AB biofilm inhibition was significantly higher in the D, E, and F groups than in the A, B, and C groups.
Myrtenol significantly reduced the air-liquid interface ring formation in glass tubes. It also effectively inhibited the attachment of BP-AB strains on polystyrene surfaces as shown by crystal violet staining. Microscopy showed a significant reduction in biofilm formation with dispersed BP-AB strains. The confocal laser scanning microscopy analysis showed a significant reduction in the biofilm\'s biomass, covered surface area, and thickness. The scanning electron microscopy analysis revealed significantly fewer BP-AB aggregates on the coverslip surface. In the CompStat analysis, the biofilm\'s biomass, maximum thickness, and surface-to-volume ratio were significantly reduced. The qPCR analysis revealed a significant down-regulation of bfmR, bap, csuA/B, and ompA expression, which positively correlated with the biofilm\'s biomass, maximum thickness, and surface-to-volume ratio in BP-AB strains.
Myrtenol significantly improved the susceptibility of BP-AB to the antibiotics amikacin, piperacillin/tazobactam, cefoperazone/sulbactam, and ceftazidime.
UNASSIGNED: Myrtenol attenuates the BP-AB biofilm formation and virulence by suppressing the expression of bfmR, bap, csuA/B, and ompA.