Aquaponics被定义为一种可持续的集成系统,将鱼类水产养殖和水培植物生产结合在同一循环水回路中。最近的一项使用高通量测序(HTS)技术的研究强调,来自水培养系统的微生物群落可以控制无土莴苣培养中最有问题的病原体之一,即,无鞘腐霉。因此,这项研究旨在分离负责这种生物防治作用的微生物。基于HTS确定的最有希望的属,设计了一种从水生水中分离和测试原始生物防治剂的创新策略,以控制P。分离出细菌菌株82株,真菌菌株18株,通过桑格测序鉴定,并在体内进行筛选,以控制由Phanikerasum引起的莴苣种子的衰减。在这100个分离株中,选择了八个最有效的方法,并进一步进行了单独测试,以在莴苣生长的后期控制由相同病原体引起的根腐病。菌株SHb30(狮食鞘),G2(黄曲霉),和Chito13(偶发分枝杆菌)比Propamocarb杀真菌剂和绿线假单胞菌注册的生物防治剂降低了种子的衰减率。在根腐病生物测定中,通过施用菌株G2和Chito13可以预防莴苣的死亡率,这些菌株至少与杀菌剂或生物农药对照一样有效。在第一次生物测定中,菌株SHb30根除了莴苣疾病症状和死亡率,但不是在第二个。这些结果表明,水培养系统是原始生物防治剂的有希望的来源,HTS指导的策略可以代表识别新生物防治剂的有趣方法。
Aquaponics is defined as a sustainable and integrated system that combines fish aquaculture and hydroponic plant production in the same recirculated water loop. A recent study using high-throughput sequencing (HTS) technologies highlighted that microbial communities from an aquaponic system could control one of the most problematic pathogens in soilless lettuce culture, namely, Pythium aphanidermatum. Therefore, this study aims at isolating the microorganisms responsible for this biocontrol action. Based on the most promising genera identified by HTS, an innovative strategy for isolating and testing original biocontrol agents from aquaponic water was designed to control P. aphanidermatum. Eighty-two bacterial strains and 18 fungal strains were isolated, identified by Sanger sequencing, and screened in vivo to control damping-off of lettuce seeds caused by P. aphanidermatum. Out of these 100 isolates, the eight most efficacious ones were selected and further tested individually to control root rot disease caused by the same pathogen at a later stage of lettuce growth. Strains SHb30 (Sphingobium xenophagum), G2 (Aspergillus flavus), and Chito13 (Mycolicibacterium fortuitum) decreased seed damping-off at a better rate than a propamocarb fungicide and a Pseudomonas chlororaphis registered biocontrol agent did. In root rot bioassays, lettuce mortality was prevented by applying strains G2 and Chito13, which were at least as efficacious as the fungicide or biopesticide controls. Lettuce disease symptoms and mortality were eradicated by strain SHb30 in the first bioassay, but not in the second one. These results show that aquaponic systems are promising sources of original biocontrol agents, and that HTS-guided strategies could represent interesting approaches to identify new biocontrol agents.