■GRAS,以GAI命名,RGA,和SCR,是一类在生长发育中起关键作用的植物特异性转录因子家族,信号转导,和各种应激反应。
■为了了解香蕉GRAS基因家族的生物学功能,基于来自M.acuminata的信息,对香蕉GRAS基因家族进行了全基因组鉴定和生物信息学分析,巴比西亚娜先生,和M.路线基因组数据库。
■在本研究中,我们确认了73个马格拉斯,59MbGRAS,和58个在香蕉全基因组尺度上的MiGRAS基因,在三个香蕉基因组中鉴定出56个同源基因。香蕉GRASs可分为10个亚家族,他们的基因结构显示大多数香蕉GRASgDNA缺乏内含子。GRASs的启动子序列中含有大量与植物生长发育相关的顺式作用元件,植物激素,和逆境压力反应。还通过定量逆转录聚合酶链反应(qRT-PCR)检查了MaGRAS对低温胁迫和不同组织的7个关键成员的表达模式。microRNAs-MaGRAS靶标预测显示7个GRAS基因与5个mac-miRNAs完美互补。所有七个基因的表达在根中最低,在低温胁迫下,5个基因在叶片中的表达量最高。MaSCL27-2、MaSCL27-3和MaSCL6-1在低温胁迫下的表达明显低于对照,除MaSCL27-2在4h时略高于28°C对照外,MaSCL27-2,MaSCL27-3和MaSCL6-1的表达在24h时降至最低水平,12h,和4小时,分别。MaSCL27-4和MaSCL6-2表达间歇性上调,在24h上升到最高表达,而MaSCL22的表达变化较小,保持在控制水平的小变化。
■总之,初步推测GRAS家族在香蕉低温胁迫中具有重要作用。本研究为进一步分析香蕉GRAS基因的功能和香蕉对低温的抗性提供了理论依据。
UNASSIGNED: GRAS, named after GAI, RGA, and SCR, is a class of plant-specific transcription factors family that plays a crucial role in growth and development, signal transduction, and various stress responses.
UNASSIGNED: To understand the biological functions of the banana GRAS gene family, a genome-wide identification and bioinformatics analysis of the banana GRAS gene family was performed based on information from the M. acuminata, M. balbisiana, and M. itinerans genomic databases.
UNASSIGNED: In the present study, we identified 73 MaGRAS, 59 MbGRAS, and 58 MiGRAS genes in bananas at the whole-genome scale, and 56 homologous genes were identified in the three banana genomes. Banana GRASs can be classified into 10 subfamilies, and their gene structures revealed that most banana GRAS gDNAs lack introns. The promoter sequences of GRASs had a large number of cis-acting elements related to plant growth and development, phytohormone, and adversity stress responsiveness. The expression pattern of seven key members of MaGRAS response to low-temperature stress and different tissues was also examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The microRNAs-MaGRASs target prediction showed perfect complementarity of seven GRAS genes with the five mac-miRNAs. The expression of all seven genes was lowest in roots, and the expression of five genes was highest in leaves during low-temperature stress. The expression of MaSCL27-2, MaSCL27-3, and MaSCL6-1 was significantly lower under low-temperature stress compared to the control, except for MaSCL27-2, which was slightly higher than the 28°C control at 4 h. The expression of MaSCL27-2, MaSCL27-3, and MaSCL6-1 dropped to the lowest levels at 24 h, 12 h, and 4 h, respectively. The MaSCL27-4 and MaSCL6-2 expression was intermittently upregulated, rising to the highest expression at 24h, while the expression of MaSCL22 was less variable, remaining at the control level with small changes.
UNASSIGNED: In summary, it is tentatively hypothesized that the GRAS family has an important function in low-temperature stress in bananas. This study provides a theoretical basis for further analyzing the function of the banana GRAS gene and the resistance of bananas to cold temperatures.