未经证实:不受控制的生物膜可导致多种疾病,如龋齿,牙龈炎,和牙周炎。龋齿是由含菌斑的致病菌积聚引起的疾病,包括粪肠球菌.这些细菌感染牙齿的根管并定殖以形成生物膜。通过干扰细胞壁形成代谢来进行生物膜抑制。MurA酶在细胞壁的肽聚糖生物合成中起作用。肠球菌表面蛋白(Esp)是粪肠球菌形成生物膜的主要贡献者。此外,通过干扰群体感应(QS)系统来抑制生物膜,通过阻断自诱导明胶酶生物合成激活信息素(GBAP)来抑制明胶酶毒力因子。
未经授权:了解Piper槟榔林的潜力。化合物作为体外抗菌药物和抗粪肠球菌的抗生物膜剂。
UNASSIGNED:通过生物活性指导的色谱方法纯化化合物。通过圆盘扩散法测试抗菌活性,体外研究。在硅研究中,将化合物P.betelL.用作测试配体,并与阳性对照磷霉素进行比较,Ambuicacid,槲皮素,和Taxifolin.使用MurA的蛋白质,Esp,GBAP,明胶酶与AutodockVinaPyRx0.8对接,然后是PYMOL程序,并通过DiscoveryStudio2020程序可视化。
未经鉴定:一种抗菌化合物被鉴定为24-丙基胆固醇,可以抑制粪肠球菌ATCC29212的活性,MIC值为78.1µg/mL,MBC值为156.3µg/mL。分子对接结果显示24-丙基胆固醇与MurA的结合亲和力,ESP,GBAP,明胶酶为-7.6、-8.7、-5.3和-7.9kcal/mol。
未经证实:24-丙基胆固醇具有作为针对粪肠球菌的抗菌剂和通过对QS的计算机抑制作为抗生物膜的潜力。然而,需要进一步的体外和体内研究来确定这些化合物作为抗菌和抗生物膜的有效性。
UNASSIGNED: Uncontrolled biofilm can cause several diseases such as dental caries, gingivitis, and periodontitis. Dental caries is a disease caused by the accumulation of plaque-containing pathogenic bacteria, including Enterococcus faecalis. These bacteria infect the root canals of teeth and colonize to form biofilms. Biofilm inhibition is carried out by interfering with cell wall formation metabolism.
MurA enzyme has a role in peptidoglycan biosynthesis of cell walls. Enterococcal surface protein (Esp) is the main contributor of E. faecalis to form biofilms. In addition, inhibition of biofilms by interfering with the quorum sensing (QS) system, suppressing gelatinase virulence factors by blocking autoinducers gelatinase biosynthesis-activating pheromone (GBAP).
UNASSIGNED: Knowing the potential of Piper betel Linn. compounds as antibacterial in vitro and antibiofilm agents against E. faecalis in silico.
UNASSIGNED: The compounds were purified by a bioactivity-guided chromatographic method. Antibacterial activity was tested by disc diffusion method, in vitro studies. In silico study, compound P. betel L. was used as the test ligand and compared with positive control fosfomycin, ambuic acid, quercetin, and taxifolin. The proteins used
MurA, Esp, GBAP, and gelatinase were docking with the Autodock Vina PyRx 0.8 followed by the PYMOL program and visualized with the Discovery Studio 2020 program.
UNASSIGNED: An antibacterial compound was identified 24-propylcholesterol which can inhibit the activity of E. faecalis ATCC 29212 with MIC value of 78.1 µg/mL and MBC value of 156.3 µg/mL. Molecular docking results showed the binding affinity of 24-propylcholesterol with
MurA, ESP, GBAP, and gelatinase enzymes was -7.6, -8.7, -5.3, and -7.9 kcal/mol.
UNASSIGNED: 24-propylcholesterol has potential as an antibacterial against E. faecalis and as an antibiofilm through in silico inhibition of QS. However, further research is needed in vitro and in vivo to determine the effectiveness of these compounds as antibacterial and antibiofilm.