食源性感染仍然是国际一级的主要公共卫生问题。在埃塞俄比亚等发展中国家,这个问题变得更加严重。
本研究旨在研究沙门氏菌和志贺氏菌和肠道寄生虫的流行情况。以及埃塞俄比亚西北部冈达尔大学自助餐厅的食品处理人员对抗生素的耐药性模式和相关因素。
一项基于机构的横断面研究于2021年2月至6月在冈达大学自助餐厅进行。使用结构化问卷收集与研究参与者的社会人口统计学特征和卫生习惯相关的数据。从食品处理人员收集了总共290个粪便样品。使用培养和常规生化测试来分离沙门氏菌和志贺氏菌。湿支架,甲酸醚浓度,和KatoKatz技术用于鉴定肠道寄生虫。此外,使用纸片扩散法进行药物敏感性试验.使用SPSS版本26进行统计分析。
分析了290名食品处理人员的粪便样本,27例(9.3%)沙门氏菌和志贺氏菌均呈阳性。沙门氏菌和志贺氏菌的流行率分别为16种(5.5%)和11种(3.8%),分别。大多数分离的病原菌对四环素19耐药(70.4%),甲氧苄啶/磺胺甲恶唑19(70.4%)。耐多药志贺氏菌和沙门菌的总检出率为59.3%。此外,57(19.7%)的参与者对一种或多种肠道寄生虫呈阳性。最普遍的肠道寄生虫病是溶组织大肠杆菌/dispar22(7.6%),其次是G.Lamblia13(4.5%),和A虫11(3.8)使用厕所后不洗手(AOR:4.42,95%CI:1.57,10.56),和食用未经巴氏灭菌的牛奶(AOR:3.14,95%CI:1.65,3.96),是与沙门氏菌患病率显着相关的因素,和志贺氏菌感染。同样,上厕所后不洗手(AOR:2.19,95%CI:1.0,1.4),和食用未经巴氏灭菌的牛奶(AOR:10.4,95%CI:3.8,28.8),是与肠道寄生虫感染患病率显著相关的因素。
肠道寄生虫的患病率,沙门氏菌,志贺氏菌种类很高。因此,必须实施一项包括持续微生物监测的公共卫生政策。
Food-borne infections continue to be a major public health problem at the international level. The issue becomes more serious in developing countries like Ethiopia.
This study aimed to examine the prevalence of Salmonella and Shigella species and intestinal parasites, as well as antimicrobial resistance patterns and associated factors among food handlers at the University of Gondar cafeteria in northwest Ethiopia.
An institutional-based cross-sectional study was conducted from February to June 2021 in the University of Gondar cafeterias. Data related to the socio-demographic characteristics and hygienic practices of study participants were collected using structured questionnaires. A total of 290 stool samples were collected from food handlers. Culture and conventional biochemical tests were used to isolate the Salmonella and the Shigella species. Wet mount, Formol-ether concentration, and Kato Katz techniques were applied to identify intestinal parasites. Additionally, drug susceptibility tests were performed using the disk diffusion method. Statistical analysis was done using SPSS version 26.
Of 290 food handlers\' stool samples analyzed, Twenty-seven 27 (9.3%) were positive for both Salmonella and Shigella species. The prevalence of Salmonella and Shigella species was 16 (5.5%) and 11 (3.8%), respectively. Most of the isolated pathogens were resistant to tetracycline 19 (70.4%), and trimethoprim/sulphamethoxazole 19 (70.4%). The overall rate of multi-drug resistant Shigella and Salmonella isolate was 59.3%. Besides, Fifty-seven 57 (19.7%) of the participants were positive for one or more intestinal parasites. The most prevalent intestinal Parasitosis was E. histolytica/dispar 22 (7.6%), followed by G. lamblia 13 (4.5%), and Ascaris lumbricoides 11 (3.8) not washing hands after using the toilet (AOR: 4.42, 95% CI: 1.57, 10.56), and consuming unpasteurized milk (AOR: 3.14, 95% CI: 1.65, 3.96), were factors significantly associated with the prevalence of Salmonella, and Shigella infection. Similarly, not washing hands after using the toilet (AOR: 2.19, 95% CI: 1.0, 1.4), and consuming unpasteurized milk (AOR: 10.4, 95% CI: 3.8, 28.8), were factors significantly associated with the prevalence of intestinal parasites infection.
The prevalence of intestinal parasites, Salmonella, and Shigella species was high. Therefore, it is imperative to implement a public health policy that includes ongoing microbiological surveillance.