中心螺旋体有许多放射状轴足,每个包含轴突微管。响应于机械刺激,轴足以接近视频速率(每秒30帧)显示出快速收缩。轴收缩伴随着细胞骨架微管解聚,但是这种现象的分子机制尚未阐明。在这项研究中,我们对收缩性弧菌进行了从头转录组测序,以鉴定与微管动力学有关的基因,如快速轴收缩。转录组测序总共产生了7.15Gbp的干净读数,组装成31,771个unigenes。使用获得的基因集,我们确定了几种可能参与快速轴突收缩的微管切断蛋白,和在基因复制中发生的驱动蛋白样基因。另一方面,一些参与鞭毛形成和运动的微管运动蛋白的基因在收缩R.这表明收缩性弧菌的基因库反映了无鞭毛原生生物的形态特征。我们的转录组分析为分析收缩性R.的微管动力学的分子机制提供了基本信息。
The centrohelid heliozoan Raphidocystis contractilis has many radiating axopodia, each containing axopodial microtubules. The axopodia show rapid contraction at nearly a video rate (30 frames per second) in response to mechanical stimuli. The axopodial contraction is accompanied by cytoskeletal microtubule depolymerization, but the molecular mechanism of this phenomenon has not been elucidated. In this study, we performed de novo transcriptome sequencing of R. contractilis to identify genes involved in microtubule dynamics such as the rapid axopodial contraction. The transcriptome sequencing generated 7.15-Gbp clean reads in total, which were assembled as 31,771 unigenes. Using the obtained gene sets, we identified several microtubule-severing proteins which might be involved in the rapid axopodial contraction, and kinesin-like genes that occur in gene duplication. On the other hand, some genes for microtubule motor proteins involved in the formation and motility of flagella were not found in R. contractilis, suggesting that the gene repertoire of R. contractilis reflected the morphological features of nonflagellated protists. Our transcriptome analysis provides basic information for the analysis of the molecular mechanism underlying microtubule dynamics in R. contractilis.