经常在环境基质中发现的氟喹诺酮抗生素(废水处理厂,医院废水,工业废水和地表废水)对环境造成潜在威胁。从环境基质中降解抗生素的酶处理是一种绿色和可持续的方法。专注于此,本研究旨在降解两种常见的氟喹诺酮类新兴污染物,环丙沙星和诺氟沙星废水。漆酶中存在的铜离子三核簇具有有效去除有机微污染物(OMPs)的能力。这项研究的独特之处在于,它利用从佛罗里达P的废蘑菇废物(SMW)中提取的漆酶来降解环丙沙星和诺氟沙星,并实现最高的降解效率,调整了各种参数,例如pH(3-6),温度(30°C和50°C),和ABTS(0.05、0.6和1mM)浓度。结果表明,环丙沙星(86.12-75.94%)和诺氟沙星(83.27-65.94%)在pH4.5,温度30°C下3h内实现了最有效的降解,和2,2'-氮杂-双3-乙基苯并噻唑啉-6-磺酸(ABTS),0.05mM浓度。然而,两种抗生素在50°C下实现降解,表示漆酶的热稳定性(P.佛罗里达)。Further,通过液相色谱(LC-MS)确认了从漆酶介导的降解获得的转化产物的命运。两种抗生素都经历了脱羧,去哌啶化,脱烷基化和脱氟是漆酶介导的键断裂的结果。通过残留抗菌毒性试验监测生物降解产品的抗微生物活性(E.大肠杆菌和金黄色葡萄球菌)。发现生物降解产物是无毒的,并导致大肠杆菌和金黄色葡萄球菌的生长,通过琼脂扩散法测定。此外,在不同的pH(3-10)和温度(4-50°C)下,测定了28天的漆酶的储存稳定性。在pH4.5和温度30°C下获得最大的储存稳定性因此,利用SMW降解废水中的OMPs不仅有利于降解,而且还能重复使用SMW农业废物,阐明农业废物管理。因此,SMW是经济中OMPs生物降解和循环性的一锅解决方案。
Fluoroquinolone antibiotics frequently found in environmental matrices (wastewater treatment plants, hospital wastewater, industrial wastewater and surface wastewater) causes potential threat to the environment. Enzymatic treatment for degradation of antibiotics from environmental matrices is a green and sustainable approach. Focusing on this, this study aimed to degrade two frequently found fluroquinolone emergent pollutants, ciprofloxacin and norfloxacin from wastewater. The trinuclear cluster of copper ions present in laccase has the ability to effectively remove organic
micropollutants (OMPs). The uniqueness of this study is that it utilizes laccase enzyme extracted from spent mushroom waste (SMW) of P. florida for degradation of ciprofloxacin and norfloxacin and to achieve highest degradation efficiency various parameters were tweaked such as pH (3-6), temperature (30 °C and 50 °C), and ABTS (0.05, 0.6, and 1 mM) concentration. The results showed that the most effective degradation of ciprofloxacin (86.12-75.94%) and norfloxacin (83.27-65.94%) was achieved in 3 h at pH 4.5, temperature 30 °C, and 2,2\'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 0.05 mM concentration. Nevertheless, achieving degradation at 50 °C for both antibiotics, indicates thermostability nature of laccase (P. florida). Further, the fate of transformed products obtained from laccase mediated degradation was confirmed by liquid chromatography (LC-MS). Both the antibiotics undergo decarboxylation, depiperylyzation, dealkylation and defluorination as a result of laccase-mediated bond breakage. Anti-microbial activity of the biodegraded products was monitored by residual anti-bacterial toxicity test (E. coli and Staphylococcus aureus). The biodegraded products were found to be non-toxic and resulted in the growth of E. coli and Staphylococcus aureus, as determined by the agar-diffusion method. Moreover, the storage stability of laccase was determined for 28-day duration at varying pH (3-10) and temperature (4-50 °C). The maximum storage stability was obtained at pH 4.5 and temperature 30 °C. Therefore, utilizing SMW for the degradation of OMPs from wastewater not only benefits in degradation but also reuses SMW agro waste, shedding light on agro waste management. Thus, SMW is a one-pot solution for both OMPs biodegradation and circularity in the economy.