This research examined the impact of exogenous thermophilic bacteria and ripening agents on greenhouse gas (GHG) emission, enzyme activity, and microbial community during composting. The use of ripening agents alone resulted in a 30.9 % reduction in CO2 emissions, while the use of ripening agents and thermophilic bacteria resulted in a 50.8 % reduction in N2O emissions. Pearson\'s analysis showed that organic matter and nitrate nitrogen were the key parameters affecting GHG emissions. There was an inverse correlation between CO2 and CH4 releases and methane monooxygenase α subunit and N2O reductase activity (P<0.05). Additionally, N2O emissions were positively related to β-1, 4-N-acetylglucosaminidase, and ammonia monooxygenase activity (P<0.05). Deinococcota, Chloroflexi, and Bacteroidota are closely related to CO2 and N2O emissions. Overall, adding thermophilic bacteria represents an effective strategy to mitigate GHG emissions during composting.

Soil microbial inoculants are increasingly being explored as means to improve soil conditions to facilitate ecological restoration. In southwestern Western Australia, highly biodiverse Banksia woodland plant communities are increasingly threatened by various factors including climate change, land development and mining. Banksia woodland restoration is necessary to conserve this plant community. The use of microbial inoculation in Banksia woodland restoration has not yet been investigated. Here, we evaluated the efficacy of a commercial microbial inoculant (GOGO Juice, Neutrog Australia Pty Ltd) for improving the performance of 10 ecologically diverse Banksia woodland plant species in a pot experiment. Plants were subjected to one of two watering regimes (well-watered and drought) in combination with microbial inoculation treatments (non-inoculated and inoculated). Plants were maintained under these two watering treatments for 10 weeks, at which point plants in all treatments were subjected to a final drought period lasting 8 weeks. Plant performance was evaluated by plant biomass and allocation, gas exchange parameters, foliar carbon and nitrogen and stable isotope (δ15N and δ13C) compositions. Plant xylem sap phytohormones were analysed to investigate the effect of microbial inoculation on plant phytohormone profiles and potential relationships with other observed physiological parameters. Across all investigated plant species, inoculation treatments had small effects on plant growth. Further analysis within each species revealed that inoculation treatments did not result in significant biomass gain under well-watered or drought-stressed conditions, and effects on nitrogen nutrition and photosynthesis were variable and minimal. This suggests that the selected commercial microbial inoculant had limited benefits for the tested plant species. Further investigations on the compatibility between the microorganisms (present in the inoculant) and plants, timing of inoculation, viability of the microorganisms and concentration(s) required to achieve effectiveness, under controlled conditions, and field trials are required to test the feasibility and efficacy in actual restoration environments.

Microbial inoculation is an important strategy to reduce the supply of heavy metals (HMs) in soil-crop systems. However, the mechanisms of microbial inoculation for the availability of HMs in soil and their accumulation/transfer in crops remain unclear. Here, the inhibitory effect of inoculation with Bacillus thuringiensis on the migration and accumulation of Pb/Cd in the soil-wheat system during the whole growth period was investigated by pot experiments. The results showed that inoculation with Bacillus thuringiensis increased soil pH and available nutrients (including carbon, nitrogen, and phosphorus), and enhanced the activities of nutrient-acquiring enzymes. Dominance analysis showed that dissolved organic matter (DOM) is the key factor affecting the availability of HMs. The content of colored spectral clusters and humification characteristics of DOM were significantly improved by inoculation, which is conducive to reducing the availability of Pb/Cd, especially during the flowering stage, the decrease was 12.8 %. Inoculation decreased Pb/Cd accumulation in the shoot and the transfer from root to shoot, with the greatest decreases at the jointing and seedling stages (27.0-34.1 % and 6.9-11.8 %), respectively. At the maturity stage, inoculation reduced the Pb/Cd accumulation in grain (12.9-14.7 %) and human health risk (4.1-13.2 %). The results of Pearson correlation analysis showed that the availability of Pb/Cd was positively correlated with the humification of DOM. Least square path model analysis showed that Bacillus thuringiensis could significantly reduce Pb/Cd accumulation in the grain and human health risks by regulating DOM spectral characteristics, the availability of HMs in soil and metals accumulation/transport in wheat at different growth stages. This study revealed the inhibition mechanism of Bacillus thuringiensis on migration of Pb/Cd in a soil-wheat system from a viewpoint of a full life cycle, which offers a valuable reference for the in-situ remediation of HM-contaminated soil and the safe production of food crops in field.

Amino acids are essential organic compounds in composting products. However, the mechanism underlying the amino acid metabolism during composting remains unclear. This study aims at exploring the impacts of inoculating cellulose-degrading microbes on amino acid metabolism during composting with mulberry branches and silkworm excrements. Cellulose-degrading microbial inoculation enhanced amino acid degradation by 18%-43% by increasing protease and sucrase activities and stimulating eight amino acid degradation pathways from the initial to thermophilic phases, with Enterococcus, Saccharomonospora, Corynebacterium being the dominant bacterial genera, but stimulated amino acid production by 54% by increasing sucrase and urease activities, decreasing β-glucosidase activities, and stimulating twenty-two amino acid synthesis pathways at the mature phase, with Thermobifida, Devosia, and Cellulosimicrobium being the dominant bacterial genera. The results suggest that cellulose-degrading microbial inoculation enhances amino acid degradation from the initial to thermophilic phases and biosynthesis at the mature phase, thereby improving the quality of organic fertilizer.

Understanding large-scale composting under natural conditions is essential for improving waste management and promoting sustainable agriculture. In this study, corn straw (400 tons) and pig manure (200 tons) were composted with microbial inoculants. The thermophilic phase of composting lasted for fourteen weeks, resulting in an alkaline final product. Microbial systems with low-temperature initiation and high-temperature fermentation played a crucial role in enhancing lignocellulose degradation and humic substances (HS) formation. Adding microbes, including Rhodanobacter, Pseudomonas, and Planococcus, showed a positive correlation with degradation rates of cellulose, hemicellulose, and lignin. Bacillus, Planococcus, and Acinetobacter were positively correlated with HS formation. Microorganisms facilitated efficient hydrolysis of lignocelluloses, providing humic precursors to accelerate composting humification through phenolic protein and Maillard pathways. This study provides significant insights into large-scale composting under natural conditions, contributing to the advancement of waste management strategies and the promotion of sustainable agriculture.

Various studies have addressed the impact of microbial inoculants on the composition of the resident microbiome. How microbial inoculants impact plant metabolism and interact with the resident rhizobiota under herbivory stress remains elusive. Here, we investigated the impact of two bacterial and two fungal inoculants, inoculated as single species and as a synthetic community, on the rhizosphere microbiome and volatilome of tomato plants (Solanum lycopersicum) comparing nonstress conditions to exposed to leaf herbivory by Spodoptera exigua. Based on amplicon sequencing analysis, rhizobacterial community composition was significantly affected by all four inoculants and the magnitude of this effect was dependent on herbivory stress. Fungal community composition was altered by the microbial inoculants but independent of herbivory stress. The rhizosphere volatilome was impacted by the microbial inoculation and differences between treatments were evened under herbivory stress. Each microbial inoculant caused unique changes in the volatilome of stressed plants but also shared similar responses, in particular the enhanced production of dimethyl disulfide and benzothiazole. In conclusion, the introduction of microbial inoculants in the tomato rhizosphere caused unique as well as common changes in the rhizosphere microbiome and volatilome, but these changes were minor compared to the microbiome changes induced by herbivory stress.

This study aims to clarify the mechanisms underlying effects of inoculating cellulose and hemicellulose-degrading microorganisms on nitrous oxide (N2O) emissions during composting with silkworm excrement and mulberry branches. Inoculation with cellulose and hemicellulose-degrading microorganisms resulted in significant increases of total N2O emission by 10.4 ± 2.0 % (349.1 ± 6.2 mg N kg-1 dw) and 26.7 ± 2.1 % (400.6 ± 6.8 mg N kg-1 dw), respectively, compared to the control (316.3 ± 3.6 mg N kg-1 dw). The stimulation of N2O emission was attributed to the enhanced contribution of ammonia-oxidizing bacteria (AOB) and denitrifying bacteria to N2O production, as evidenced by the increased AOB amoA and denitrifying nirK gene abundances. Moreover, microbial inoculation stimulated N2O reduction to N2 owing to increased abundances of nosZⅠ and nosZⅠⅠ genes. These findings highlight the necessity to develop cost-effective and environmentally friendly strategies to reduce N2O emissions when cellulose and hemicellulose-degrading microorganisms are inoculated during composting.

This study aimed to explore the impact of co-inoculating phosphate-solubilizing bacteria (PSB) and phosphate accumulating bacteria (PAB) on phosphorus forms transformation, microbial biomass phosphorus (MBP) and polyphosphate (Poly-P) accumulation, bacterial community composition in composting, using high throughput sequencing, PICRUSt 2, network analysis, structural equation model (SEM) and random forest (RF) analysis. The results demonstrated PSB-PAB co-inoculation (T1) reduced Olsen-P content (1.4 g) but had higher levels of MBP (74.2 mg/kg) and Poly-P (419 A.U.) compared to PSB-only (T0). The mantel test revealed a significantly positive correlation between bacterial diversity and both bioavailable P and MBP. Halocella was identified as a key genus related to Poly-P synthesis by network analysis. SEM and RF analysis showed that pH and bacterial community had the most influence on Poly-P synthesis, and PICRUSt 2 analysis revealed inoculation of PAB increased ppk gene abundance in T1. Thus, PSB-PAB co-inoculation provides a new idea for phosphorus management.

Due to limited urban green spaces and catchments, researchers are exploring the capacity of vertical greenery systems (VGSs) in stormwater management as complementary strategies. While the literature acknowledges the significant impacts of vegetated roof substrates on stormwater, comparing the stormwater management capacities of organic and non-organic substrates for VGSs remains largely unexplored. It is thus essential to gather empirical evidence to enhance the stormwater management capacity of VGSs. Here, we report on the impact of installation factors (substrate type and plant growth-promoting microbe (PGPM) inoculation) and environmental factors (simulated rainwater quantity and substrate moisture) of an innovative VGS on the concentrations and total loads of 15 elements (N, P, Al, V, Cr, Fe, Mn, Co, Ni, Cu, Zn, As, Se, Cd, and Pb) in the runoff. Results showed that substrate type was the most influential factor: concentrations and total loads were significantly higher from a reed-based substrate with high organic matter than from a sandy loam substrate. Substrate type also had profound interactive effects with other factors. For instance, PGPM inoculation significantly reduced the total loads of As, Cr, N, Ni, and Se, regardless of substrate type, and reduced the total loads of Cd, Co, Cu, Fe, Mn, and Pb in the reed-based substrate only. In addition, PGPM inoculation primarily reduced total loads, yet had little effect on concentrations. Substrate type also interacted with simulated rainwater quantity and substrate moisture: for example, in the reed-based substrate, a higher simulated rainwater quantity reduced concentrations but increased total loads, while concentrations and total loads remained constantly low from the sandy loam substrate under various simulated rainwater quantities. High antecedent substrate moisture increased both concentrations and total loads for most of the elements. We conclude that leaching from VGSs can be contained via substrate selection, maintenance of substrate moisture, and beneficial PGPM inoculation.

The challenge of managing agricultural phosphorus (P) in saline regions entails both reducing leaching for environmental protection and maintaining soil available P levels for crop production, which could be achieved through functional microorganisms that can facilitate P transformation processes like P assimilation, inorganic P solubilization, and organic P mineralization. In this study, we proposed an integrated utilization of phosphorus-accumulating bacteria (PAB) and phosphorus-solubilizing bacteria (PSB) to reach the goal of alleviating P leaching while improving soil available P levels. The study conducted a microcosm experiment that combined a soil column test, soil incubation, and pot experiment to evaluate the effect of bacterial inoculants on soil P leaching, soil P availability, and plant P accumulation. The results showed that the application of PAB reduced 22.6 % of dissolved P leaching through the absorption of labile phosphate in the soil, and 17.3 % of particulate P leaching through the promoted soil aggregation. The integrated inoculation of PSB and PAB synergistically improved soil available P content by 18.3 % through the mineralization of soil organic P, and remarkably boosted wheat growth and its P accumulation. Microbial community analysis revealed that the integrated microbial treatment decreased the diversity of soil bacterial community and increased the abundance of native microbial species, i.g. Lysobacter and Ramlibacter, which were positively correlated with soil available P content and alkaline phosphatase level. In conclusion, the integrated microbial strategy based on halotolerant PAB and PSB has great potential for sustainable P management in saline areas and agricultural activities.