乳腺癌仍然是全球女性发病和死亡的首要主要原因之一。在目前的研究中,miR-590-5p和配对同源域转录因子2(PITX2)对增殖的影响,入侵,迁移,通过Wnt-β-catenin信号通路研究人乳腺癌的上皮-间质转化(EMT)。从KEGG数据库获得乳腺癌相关基因和相关信号通路。预测了PITX2调节微小RNA。为了定义miR-590-5p影响乳腺癌进展的作用,探讨了miR-590-5p与PITX2之间的相互作用;增殖,入侵,在miR-590-5p过表达或沉默后,检测到裸鼠的迁移能力以及肿瘤生长和转移。确定PITX2与乳腺癌具有相关性,并且选择miR-590-5p用于进一步分析。PITX2,Wnt-1,β-连环蛋白,N-钙黏着蛋白,和波形蛋白都显示更高的水平,而miR-590-5p和E-cadherin在乳腺癌组织中的表达低于癌旁正常组织。miR-590-5p或si-PITX2过表达后,E-cadherin的表达明显增加,Wnt-1,β-catenin,N-钙黏着蛋白,还有波形蛋白,以及抑制细胞增殖,入侵,迁移,转移,并观察到EMT。这项研究提供的证据表明,过表达的miR-590-5p的转染可以减轻乳腺癌的影响,证明了抑制细胞增殖过程的能力。迁移,通过抑制PITX2的表达和Wnt-β-catenin途径的激活,以及侵袭和EMT。
Breast cancer remains one of the foremost primary causes of female morbidity and mortality worldwide. During the current study, the effect of miR-590-5p and paired-like homeodomain transcription factor 2 (PITX2) on proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) of human breast cancer via the Wnt-β-catenin signaling pathway was investigated. Breast cancer-related genes and related signaling pathways were obtained from KEGG database. The PITX2 regulatory microRNA was predicted. To define the contributory role by which miR-590-5p influences the progression of breast cancer, the interaction between miR-590-5p and PITX2 was explored; the proliferation, invasion, and migration abilities as well as the tumor growth and metastasis in nude mice were detected following the overexpression or silencing of miR-590-5p. PITX2 was determined to share a correlation with breast cancer and miR-590-5p was selected for further analysis. PITX2, Wnt-1, β-catenin, N-cadherin, and vimentin all displayed higher levels, while miR-590-5p and E-cadherin expression were lower among breast cancer tissues than in the adjacent normal tissue. After overexpression of miR-590-5p or si-PITX2, the expression of E-cadherin was markedly increased, decreases in the expression of Wnt-1, β-catenin, N-cadherin, and vimentin, as well as inhibited cell proliferation, invasion, migration, metastasis, and EMT were observed. This study provides evidence suggesting that the transfection of overexpressed miR-590-5p can act to alleviate the effects of breast cancer demonstrating an ability to inhibit the processes of cell proliferation, migration, and invasion as well as EMT by suppressing the expression of PITX2 and activation of the Wnt-β-catenin pathway.