The aim of this work was to provide evidence on the presence of microplastics (MPs) in regurgitated Yellow-legged Gull pellets (n = 18) from Sfax salina (south-eastern Tunisia). This artificial area is subject to high anthropogenic pressure and hosts Yellow-legged Gulls, which are at the top of the trophic chain and can be used as sentinel species to monitor litter in the environment, including plastic pollution. The total number of MPs found in the samples was 309, 63.8 % fibres (4.95 ± 3.51 MPs/g) and 36.2 % fragments (2.87 ± 1.74 MPs/g). Micro-FTIR analysis evidenced that a large proportion of the fibres was attributed to artificial cellulose (40.7 %). Ethylene vinyl acetate (EVA) and polyethylene (PE) were found in the fragments.

Microplastic (MPs) pollution is a current global concern that is affecting all environmental compartments and food sources. In this work, anthropogenic particles occurrence (MPs and natural and synthetic cellulosic particles), have been determined in 73 beverages packed in different containers. Overall, 1521 anthropogenic particles were found, being the lowest occurrence in water samples (7.2 ± 10.1 items·L-1) while beer had the highest (95.5 ± 91.8 items·L-1). Colourless/white particles were the most detected followed by blue and red colours. The highest mean size was 783 ± 715 μm in soft drinks. Cellulosic, both natural and semisynthetic particles, were the composition mostly found but regarding plastic polymers, it was polyester. Phenoxy resin particles from the can coatings were also identified in all metal containers which indicates that leaching from the packaging may be happening. The total estimated daily intake were 0.077 and 0.159 items·kg-1 body weight (b.w.)·day-1 for children and adult population, respectively.

Litter pollution is a growing concern, including for Antarctica and the species that inhabit this ecosystem. In this study, we investigated the microplastic contamination in three seal species that inhabit the Western Antarctic Peninsula: crabeater (Lobodon carcinophaga), leopard (Hydrurga leptonyx) and Weddell (Leptonychotes weddellii) seals. Given the worldwide ubiquity of this type of contaminant, including the Southern Ocean, we hypothesized that the three seal species would present anthropogenic debris in their feces. We examined 29 scat samples of crabeater (n = 5), leopard (n = 13) and Weddell (n = 11) seals. The chemical composition of the items found were identified using micro-Raman and micro-FTIR spectroscopies. All the samples of the three species presented anthropic particles (frequency of occurrence - %FO - 100 %). Fibers were the predominant debris, but fragments and filaments were also present. Particles smaller than 5 mm (micro debris) were predominant in all the samples. Leopard seals ingested significantly larger micro-debris in comparison with the other seal species. The dominant color was black followed by blue and white. Micro-Raman and micro-FTIR Spectroscopies revealed the presence of different anthropogenic pigments such as reactive blue 238, Indigo 3600 and copper phthalocyanine (blue and green). Carbon black was also detected in the samples, as well as plastic polymers such as polystyrene, polyester and polyethylene terephthalate (PET), polyamide, polypropylene and polyurethane These results confirm the presence of anthropogenic contamination in Antarctic seals and highlight the need for actions to mitigate the effects and reduce the contribution of debris in the Antarctic ecosystem.

Plastics account for a large proportion of domestic waste. However, micro-plastics will be produced after the plastic is incinerated. The purpose of this study is to find out the change rule of micro-plastics produced during incineration under different conditions. Combining micro-FTIR and PCA algorithm is a good tool to identify the micro-plastics. The PE, PP and PVC micro-plastics are distinguished using PCA-FTIR spectra. The results show different incineration conditions significantly affect the output of micro-plastics. The yield of micro-plastics increases with increasing temperature for both PP and PVC. And the yield of micro-plastics decreases with the increase in flow rate. The maximum amount of micro-plastics is produced by PE, which is 6.62 × 103 after 1 g PE incineration. The yield of micro-plastics in the co-incineration of PE and PP, as well as PE and PVC, significantly increased to 1.42 and 1.89 times of the calculated values, respectively. The nano-particles are also observed. The FTIR and EDS results show that the nano-particles are the products of incineration of plastics, including partly CH bond and unburned carbon, tar and ash.

Microplastics (MPs) in the atmosphere can undergo long-range transport from emission regions to pristine terrestrial and oceanic ecosystems. Due to their inherent toxic and hazardous characteristics, MPs pose serious risks to both human well-being and the equilibrium of ecosystem. The present study outlines the comprehensive characterization, spanning physical and chemical attributes of MPs associated with atmospheric aerosols. Total suspended particulates (TSPs) were collected on a quartz fibre filter by operating a high-volume sampler for 24 h during distinct years (March, 2016 and November, 2020) at a coastal location in the northeast Arabian Sea. Subsequent to the sampling, a series of techniques were applied including density separation. The assessment and scrutiny of the MPs was carried out using stereo-zoom microscopy with supplementary validation using advanced fluorescence microscopy for enhanced precision in identification. Our comparative assessment suggests peroxide treatment followed by density separation could be a robust procedure for the definitive identification and characterization of MPs in the atmosphere. Average total abundance of MPs was found to be 1.30 ± 0.14 n/m3 in 2016 and 1.46 ± 0.12 n/m3 in 2020 with fibres, fragments and films having similar relative contributions (41 %, 31 %, 28 % in 2016 and 40 %, 35 %, 25 % in 2020). Fibres were found to be dominant morphotype followed by fragments and films over the coastal region of the Arabian Sea. In order to unravel the detailed chemical nature of these MPs, spectral analysis using μ-FTIR was carried out. The outcome of the analysis showed prevailing polymers as polyvinyl chloride and polymethyl methacrylate (50545 %) as dominant polymers followed by polyester (15 %), styrene butyl methacrylate (11 %), and polyacetal (9 %). MPs present in the vicinity of the Arabian Sea have potential to supply nutrients and toxicants, consequently can contribute to the modulation of the surface water biogeochemical processes.

Road dust is one of the environment\'s most important microplastic and plastic additive sources. Traffic vehicles and the wear of tires can release these emerging contaminants, which can be resuspended in the air and washed off by stormwater runoff. In this study, a concurrent quantification and chemical characterization of additives, plasticizers, natural and non-plastic synthetic fibers (APFs), and small microplastics (SMPs, <100 µm) in samples of highway road dust (HWRD) was performed. The sampling procedure was optimized, as well as pretreatment (extraction, purification, and filtration) and analysis via micro-FTIR. The average length of the SMPs was 88 µm, while the average width was 50 µm. The highest abundance of SMPs was detected in HWRD 7 (802 ± 39 SMPs/g). Among the polymers characterized and quantified, vinyl ester and polytetrafluoroethylene were predominant. APFs\' average particle length was 80 µm and their width was 45 µm, confirming that both of these emerging pollutants are less than 100 µm in size. Their maximum concentration was in RD7, with 1044 ± 45 APFs/g. Lubricants and plasticizers are the two most abundant categories, followed by vulcanizing agents, accelerators, and pre-vulcanizing retarders derived mainly from tires. A potential relationship between APFs and SMPs in the different seasons was observed, as their concentration was lower in summer for both and higher in winter 2022. These results will be significant in investigating the load of these pollutants from highways, which is urgently necessary for more accurate inclusion in emission inventories, receptor modeling, and health protection programs by policymakers, especially in air and water pollution policies, to prevent risks to human health.

Despite the omnipresence of microplastics (MPs), the studies around the western continental shelf of Indian Ocean (Eastern Arabian Sea-EAS) are uncovered and understudied. Thus, the present study was focused to understand the spatial distribution, characterization and risk assessment of MPs in sediment across seven coastal transects (10 to 50 m) all along the EAS shelf. The highest MPs concentration (MPs/kg d.w.) was detected in the northern EAS (NEAS; 2260 ± 1050) followed by central (CEAS; 1550 ± 1012) and southern (SEAS; 1300 ± 513) shelves. Among all distinct locations, the highest concentration of MPs (2500 ± 1042) was detected in the north coastal sediments off Mumbai, followed by off Mangalore (1480 ± 1169) in the center and off Kochi (1350 ± 212) in the south. MPs were found in the form of fibres, fragments and films with a predominance of fibres (~70-80 %). Approximately 74.6 % of the total MPs were in the size range of 300 μm to 5 mm. The surface of detected MPs was rough, irregular, and mechanical weathering features such as pits, grooves also observed and spotted with bacterial community structures. Polypropylene (PP; 34 %), polyisoprene (PIP; 19 %), butyl rubber (18 %), and low-density polyethylene (LDPE; 13 %) were dominant polymers. The pollution load index highlighted minor risk while the polymer hazard index exhibited a hazard level of V. Litter discharge, fishing activities, and active marine navigation are among the many high-risk sources of plastic contamination in this region. Due to the prevailing winds, currents, low sea surface height, and high precipitation, the conditions in the EAS are favorable for the accumulation of both sea-based and land-based particles. Hence, this study provides novel insights into the potential risks posed by MP to the IO rim and associated marine ecosystem which will enhance our knowledge of the ecological implications and consequences of MP pollution, ultimately aiding in developing effective management and mitigation strategies.

The ubiquity of microplastics (MPs) in the various environment is of increasing concern. Although micro Fourier Transform Infrared Spectroscopy (μ-FTIR) represents an ideal method for the detection of MPs, this technique lacks a standardized mode to be followed for MPs in diverse environmental matrices. The study focused on the optimization, application, and validation of μ-FTIR techniques for the identification of smaller-sized MPs (20 μm-1 mm). In order to assess the validity of the various detection modes in μ-FTIR (reflection and transmission), a confirmatory test with known standard polymers, viz., polyethylene (PE), polypropylene (PP), polystyrene (PS), polyamide (PA), and polyvinyl chloride (PVC) were executed. Subsequently, for the validation and accuracy of the method, the polymer spectra of standard polymers acquired in μ-FTIR (smaller-sized) were compared with spectra of larger-sized particles (same standards) in FTIR-ATR (Attenuated Total Reflectance). The spectra were comparable and highlighted the similar pattern of the polymeric composition. The spectral quality and matching score (>60 %) with the reference library was taken into account to accentuate the authenticity of the different methods. This study highlighted the reflection mode (particularly diffuse reflection) as more effective for the quantification of smaller-sized MPs in complex environmental samples. The same method was successfully applied to a representative environmental sample (sand), supplied by the EURO-QCHARM for inter-laboratory study. Two polymers (PE and PET) were correctly identified out of three spiked polymers (PE, PET, and PS) in the given sample. Similarly, in terms of matching algorithms, the results for diffuse reflection (PE-71.7 % and PET-89.1 %) were found satisfactory as compared to micro-ATR (PE-67 % and PET-63.2 %) reflection mode. Overall, this study illustrates an extensive perspective of different μ-FTIR techniques, recommending the most reliable, easy, and non-destructive method to unambiguously characterize diverse types of polymers of smaller MPs in complex environmental matrices.

BACKGROUND: Escherichia coli cells are the most frequently used hosts in recombinant protein production processes and mainly require molecules such as IPTG or pure lactose as inducers of heterologous expression. A possible way to reduce the production costs is to replace traditional inducers with waste materials such as cheese whey permeate (CWP). CWP is a secondary by-product generated from the production of the valuable whey proteins, which are obtained from ultrafiltration of cheese whey, a main by-product of the dairy industry, which is rich in lactose.
RESULTS: The effects of CWP collected from an Italian plant were compared with those of traditional inducers on the production of two model proteins (i.e., green fluorescent protein and the toxic Q55 variant of ataxin-3), in E. coli BL21 (DE3) cells. It was found that the high lactose content of CWP (165 g/L) and the antioxidant properties of its micronutrients (vitamins, cofactors and osmolytes) sustain production yields similar to those obtained with traditional inducers, accompanied by the improvement of cell fitness.
CONCLUSIONS: CWP has proven to be an effective and low-cost alternative inducer to produce recombinant proteins. Its use thus combines the advantage of exploiting a waste product with that of reducing the production costs of recombinant proteins.