一种细菌菌株,指定NLS-7T,是通过在甲烷氧化条件下富集垃圾填埋场覆盖土壤而分离的。NLS-7T菌株是革兰氏染色阴性,非运动棒,大约0.8µm宽,1.3µm长。基于16SrRNA基因测序的系统发育分析将其置于Methylocystis属中,它的近亲是M.hirsuta,M.silviterrae和M.Rosea,序列相似性分别为99.9%、99.7%和99.6%。然而,与所有近亲相比,平均核苷酸同一性和平均氨基酸同一性值低于95%阈值,数字DNA-DNA杂交值介于20.9和54.1%之间,这表明NLS-7T菌株代表了新物种。基因组测序产生431万个读段,基因组组装导致产生244个重叠群,总组装长度为3820957bp(N50,37735bp;L50,34)。基因组完整性为99.5%,污染为3.98%。它能够在甲烷和甲醇上生长。它在30°C下在pH6.5和7.0之间最佳生长。菌株NLS-7T能够在大气中固定氮,并且可以使用铵(如NH4Cl),l-天冬氨酸,l-精氨酸,酵母提取物,硝酸盐,l-亮氨酸,l-脯氨酸,l-蛋氨酸,L-赖氨酸和L-丙氨酸作为氮源。主要的脂肪酸是C18:1ω8c和C18:1ω7c。基于这项多相分类学研究,菌株NLS-7T代表了一种新的家属种,其名称为Methylocystissuflitaesp。11月。是提议的。菌株类型为NLS-7T(=ATCCTSD-256T=DSM112294T)。菌株NLS-7T的16SrRNA基因和基因组序列已分别以登录号ON715489和GCA_024448135.1保藏在GenBank中。
A bacterial strain, designated NLS-7T, was isolated through enrichment of landfill cover soil in methane-oxidizing conditions. Strain NLS-7T is a Gram-stain negative, non-motile rod, approximately 0.8 µm wide by 1.3 µm long. Phylogenetic analysis based on 16S rRNA gene sequencing places it within the genus Methylocystis, with its closest relatives being M. hirsuta, M. silviterrae and M. rosea, with 99.9, 99.7 and 99.6 % sequence similarity respectively. However, average nucleotide identity and average amino acid identity values below the 95 % threshold compared to all the close relatives and digital DNA-DNA hybridization values between 20.9 and 54.1 % demonstrate that strain NLS-7T represents a novel species. Genome sequencing generated 4.31 million reads and genome assembly resulted in the generation of 244 contigs with a total assembly length of 3 820 957 bp (N50, 37 735 bp; L50, 34). Genome completeness is 99.5 % with 3.98 % contamination. It is capable of growth on methane and methanol. It grows optimally at 30 °C between pH 6.5 and 7.0. Strain NLS-7T is capable of atmospheric dinitrogen fixation and can use ammonium (as NH4Cl), l-aspartate, l-arginine, yeast extract, nitrate, l-leucine, l-proline, l-methionine, l-lysine and l-alanine as nitrogen sources. The major fatty acids are C18:1 ω8c and C18:1 ω7c. Based upon this polyphasic taxonomic study, strain NLS-7T represents a novel species of the genus Methylocystis, for which the name Methylocystis suflitae sp. nov. is proposed. The type strain is NLS-7T (=ATCC TSD-256T=DSM 112294T). The 16S rRNA gene and genome sequences of strain NLS-7T have been deposited in GenBank under accession numbers ON715489 and GCA_024448135.1, respectively.