■耐甲氧西林葡萄球菌(MRS)严重威胁动物和人类健康。反复使用抗生素可使细菌对几种抗生素产生耐药性,并成为多重耐药(MDR)。犬脓皮病,狗常见的皮肤状况,主要是由葡萄球菌引起的,包括夫人。在所有犬类人群中检测这种感染对于制定适当的预防计划至关重要。这项研究估计了患病率,抗菌谱,孔敬一家转诊动物医院的犬患者MRS的危险因素,泰国。
皮肤拭子和相关信息是从2019年9月至2020年9月到医院就诊的56只客户拥有的狗收集的。使用自动化系统(VITEK®2)对葡萄球菌菌落进行分子鉴定和抗生素敏感性测试。还使用多重聚合酶链反应(PCR)和测序对这些菌落进行了遗传鉴定。mecA基因,编码耐甲氧西林,使用单纯PCR检测。采用logistic回归和卡方检验分析MRS感染的危险因素及其与MRS感染的关系。分别。
■发现MRS的患病率为35.7%(20/56只狗)。按物种,在104个分离株中的24个(23.1%)中发现了耐甲氧西林假中间葡萄球菌,所有样品均为MDR。过去6个月接受全身抗生素治疗是MRS感染的主要危险因素(p<0.05;比值比(OR)>1)。除了MRS分离株,在对甲氧西林敏感的葡萄球菌分离株中也检测到mecA基因.这可能是因为blaI的高表达,和c-di-AMP环化酶DacA的突变,Rela,和Fem蛋白。
■在研究人群中观察到MRS和MDR的高患病率,这可能是由于所有者不当使用抗生素和耐药基因的水平转移。
UNASSIGNED: Methicillin-resistant Staphylococci (MRS) seriously threatens animal and human health. Repeated antibiotic use allows the bacteria to develop resistance to several antibiotic classes and become multidrug-resistant (MDR). Canine pyoderma, a common skin condition in dogs, is mainly caused by Staphylococci, including MRS. Detecting this infection in all canine populations is crucial to develop a proper preventive plan. This study estimated the prevalence, antibiogram, and risk factors of MRS in canine patients at a referral animal hospital in Khon Kaen, Thailand.
UNASSIGNED: Skin swabs and relevant information were collected from 56 client-owned dogs that visited the hospital from September 2019 to September 2020. Staphylococci colonies were subjected to molecular identification and antibiotic susceptibility tests using an automated system (VITEK® 2). These colonies were also genetically identified using multiplex-polymerase chain reaction (PCR) and sequencing. The mecA gene, encoding methicillin resistance, was detected using simplex-PCR. The risk factors of MRS infection and their association with MRS infection were analyzed using logistic regression and the Chi-square test, respectively.
UNASSIGNED: The prevalence of MRS was found to be 35.7% (20/56 dogs). By species, methicillin-resistant Staphylococcus pseudintermedius was found in 24 of 104 isolates (23.1%), and all samples were MDR. Receiving systemic antibiotics in the past 6 months was a major risk factor associated with MRS infection (p < 0.05; odds ratio (OR) > 1). In addition to the MRS isolates, the mecA gene was also detected in methicillin-susceptible Staphylococci isolates. This might be because of the high expression of blaI, and mutations in c-di-AMP cyclase DacA, RelA, and Fem proteins.
UNASSIGNED: A high prevalence of MRS and MDR was observed in the studied population, which might be potentially due to improper antibiotic use by the owners and horizontal transfer of drug-resistance genes.