■白色斑点的花巾(Protaetiabrevitarsisseulensis),在亚洲国家广泛分布,传统上用于东方医学。然而,在繁殖过程中,其幼虫容易受到绿色muscardine病(由绿僵菌引起)的严重损害。这项研究的目的是表征从淡水中分离出的淡黄色芽孢杆菌TJS119,并研究其作为生物防治昆虫的潜力。
■TJS119是从大韩民国的淡水样品中获得的,被归类为维氏芽孢杆菌。我们评估了其体外抗真菌作用,对细菌全基因组进行测序,挖掘负责次级代谢产物合成的基因,进行次级代谢产物分析超高效液相色谱-质谱(UPLC-MS/MS),并进行了生物测定以确定绿色muscardine疾病控制能力。
■VelezensisTJS119在体外抑制了M.anisopliae的菌丝生长。VelezensisTJS119基因组的大小估计为3,890,913bp,GC含量为46.67%,编码序列为3,750。在基因组中鉴定了具有抗真菌活性的次级代谢物的生物合成基因簇。脂肽,包括由TJS119分泌的fengycin表现出抗真菌活性。与对照相比,将TJS119应用于针对绿色muscardine病的生物防治使白色斑点花金龟子的生存力提高了94.7%。
■这些结果表明B.velezensisTJS119是昆虫病原体的潜在生物防治剂。
UNASSIGNED: The white-spotted flower chafer (Protaetia brevitarsis seulensis), which is widely distributed in Asian countries, is traditionally used in oriental medicine. However, its larvae are prone to severe damage by green muscardine disease (caused by Metarhizium anisopliae) during breeding. The aim of this study was to characterize Bacillus velezensis TJS119, which has been isolated from freshwater, and investigate its potential as a biocontrol agent against M. anisopliae in insects.
UNASSIGNED: TJS119 was obtained from freshwater samples in the Republic of Korea and was classified as B. velezensis. We evaluated its in vitro antifungal effect, sequenced the bacterial whole genome, mined genes responsible for the synthesis of secondary metabolites, performed secondary metabolite analysis Ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS), and conducted bioassays for determining green muscardine disease control ability.
UNASSIGNED: Bacillus velezensis TJS119 inhibited the mycelial growth of M. anisopliae in vitro. The size of the B. velezensis TJS119 genome was estimated to be 3,890,913 bp with a GC content of 46.67% and 3,750 coding sequences. Biosynthetic gene clusters for secondary metabolites with antifungal activity were identified in the genome. Lipopeptides, including fengycin secreted by TJS119 exhibit antifungal activity. Application of TJS119 for the biocontrol against green muscardine disease increased the viability of white-spotted flower chafer by 94.7% compared to the control.
UNASSIGNED: These results indicate that B. velezensis TJS119 is a potential biocontrol agent for insect pathogens.