■番茄,茄子,是最重要的园艺作物之一,可以在全球范围内新鲜或加工后食用。番茄叶矿工(Tutaabsoruta)是对番茄植物最具破坏性的害虫之一,因为它在植物的叶肉组织中具有采雷性质。真菌昆虫病原体可以作为渐近线自然存在于植物中。本研究旨在通过人工接种来检测番茄植株中球孢白僵菌和绿僵菌的内生定植及其对Tutasescaluta的毒力影响。
■通过不同的人工接种技术选择具有最高的发芽百分比和对T.absoruta的毒力的分离株用于番茄植物内的内生评价。
■这项研究表明,发芽百分比最高且对Tutaabsoruta具有毒性的分离株有可能在番茄植株上定殖。结果表明,在接种后第7天,以1×108分生孢子/ml的浓度,通过分离株K-61,达到了绝对Tuta幼虫的最大死亡率(97.5%).然而,球孢白僵菌分离株APPRC-27在通过直接接触法接种后10天记录到最高的累积死亡率(100%).在接种后7天,使用叶片喷雾技术,分离株APPRC-27(76.67%)记录了最高的内生定植,但接种28天后下降到11.67%。在幼苗接种技术的情况下,分离株K-61接种28天后,番茄根组织中的内生菌定植最高。
■这项研究表明,喷叶接种技术是最有效的方法,接着是幼苗接种,用于在番茄植物组织中部署球孢白僵菌和绿僵菌内生菌。因此,毒力球孢白僵菌和绿僵菌,如果作为内生菌部署,是控制Tutaabsoruta的有前途的生物制剂。
UNASSIGNED: The tomato, Solanum lycopersicum L., is one of the most important horticultural crops that can be consumed fresh or after being processed worldwide. The tomato leaf miner (Tuta absoluta) is one of the most devastating pest to tomato plants due to its mine-feeding nature in the mesophyll tissue of the plant. Fungal entomopathogens can exist naturally in plants as an asymptote. This study aimed to detect the endophytic colonization of Beauveria bassiana and
Metarhizium robertsii within tomato plants via artificial inoculation and their virulence effects on Tuta absoluta.
UNASSIGNED: Isolates with the highest percent germination and virulence against T. absoluta were selected for endophytic evaluation within tomato plants by different artificial inoculation techniques.
UNASSIGNED: This study revealed that, isolates with the highest percent germination and virulent to Tuta absoluta had the potential to colonize tomato plants. The result showed that, the maximum mortality rate (97.5%) of Tuta absoluta larvae was achieved by
Metarhizium robertsii isolate K-61 at a concentration of 1x10 8conidial/ml at 7 days post inoculated. However, the highest cumulative mortality (100%) was recorded by Beauveria bassiana isolate APPRC-27 at 10 days post inoculated through the direct contact method. The highest endophytic colonization was registered by isolate APPRC-27 (76.67%) at 7 days post-inoculated using the leaf spray technique, but it declined to 11.67% after 28 days of inoculated. In the case of the seedling inoculation technique, the highest endophytic colonization was obtained in the root tissues of tomatoes at 28 days of inoculated by isolate K-61.
UNASSIGNED: This study revealed that the leaf spray inoculation technique was the most effective method, followed by seedling inoculation, for the deployment of Beauveria bassiana and
Metarhizium robertsii endophytes in tomato plant tissues. Therefore, virulent Beauveria bassiana and
Metarhizium robertsii, are promising bioagents for the control of Tuta absoluta if deployed as endophytes.